Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/107175
Title: Validation of an LC-MS/MS Method for the Quantification of Caffeine and Theobromine Using Non-Matched Matrix Calibration Curve
Authors: Mendes, Vera M. 
Coelho, Margarida 
Tomé, Ângelo R. 
Cunha, Rodrigo A. 
Manadas, Bruno 
Keywords: caffeine; LC-MS/MS; non-matched matrix calibration curve
Issue Date: 7-Aug-2019
Publisher: MDPI
Project: This work was financed by the European Regional Development Fund (ERDF) through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia, I.P., under projects: PTDC/NEU-NMC/0205/2012, POCI-01-0145-FEDER-007440 (ref.; UID/NEU/04539/2019), POCI-01-0145-FEDER-016428 (ref.: SAICTPAC/0010/2015), POCI-01-0145-FEDER-016795 (ref.: PTDC/NEU-SCC/7051/2014), POCI-01-0145-FEDER-029311 (ref.: PTDC/BTM-TEC/29311/2017), POCI-01-0145-FEDER-30943 (ref.: PTDC/MEC-PSQ/30943/2017) and PTDC/MED-NEU/27946/2017; and by The National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). MC was supported by PhD fellowship PD/BD/135178/2017, co-financed by the European Social Fund (ESF) through the POCH - Programa Operacional do Capital Humano and national funds via FCT. 
Serial title, monograph or event: Molecules
Volume: 24
Issue: 16
Abstract: Caffeine is one of the most widely consumed psycho-stimulants. The study of the beneficial effects of caffeine consumption to decrease the risk of developing several neuropsychiatric pathologies is receiving increasing attention. Thus, accurate and sensitive methods have been developed, mainly by LC-MS/MS, in order to quantify caffeine and its metabolites. These quantifications of caffeine and its metabolites by LC-MS/MS require a considerable effort to select or find a surrogate matrix, without the compounds of interest, to be used in the calibration curves. Thus, we evaluated the possibility of using calibration curves prepared in solvent instead of calibration curves prepared in human plasma. Results show that the calibration curves prepared in solvent and in human plasma were similar by comparing their slopes and interceptions, and the accuracy and precision were within the limits of acceptance for both calibration curves. This work demonstrates that, by using internal standards, it is possible to use a calibration curve in solvent instead of a calibration curve in plasma to perform an accurate and precise quantification of caffeine and theobromine.
URI: https://hdl.handle.net/10316/107175
ISSN: 1420-3049
DOI: 10.3390/molecules24162863
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
FCTUC Química - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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