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Title: Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity
Authors: Paula, Anabela 
Laranjo, Mafalda 
Marto, Carlos Miguel 
Abrantes, Ana Margarida 
Casalta-Lopes, João 
Gonçalves, Ana Cristina 
Ribeiro, Ana Bela Sarmento 
Ferreira, Manuel M. 
Botelho, Maria Filomena 
Carrilho, Eunice 
Keywords: biocompatibility; biomaterials; cytotoxicity; dentinogenesis; odontoblast; pulp capping
Issue Date: 11-Apr-2019
Publisher: MDPI
Project: GAI 2013 (Faculdade de Medicina da Universidade de Coimbra) 
FCT - Strategic Project PEst-C/SAU/UI3282/2013 e UID/NEU/04539/2013] 
Serial title, monograph or event: Materials
Volume: 12
Issue: 7
Abstract: (1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life® induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot®MTA and Biodentine™ induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine™. (4) Conclusions: The results of WhiteProRoot®MTA confirm its indication for these therapies, justifying its recognition as the "gold standard". Biodentine™ may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.
ISSN: 1996-1944
DOI: 10.3390/ma12071184
Rights: openAccess
Appears in Collections:I&D IBILI - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais

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