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Title: | Sargassum muticum and Osmundea pinnatifida Enzymatic Extracts: Chemical, Structural, and Cytotoxic Characterization | Authors: | Rodrigues, Dina Costa-Pinto, Ana R. Sousa, Sérgio Vasconcelos, Marta W. Pintado, Manuela M. Pereira, Leonel Rocha-Santos, Teresa A. P. Costa, João P. da Silva, Artur M. S. Duarte, Armando C. Gomes, Ana M. P. Freitas, Ana C. |
Keywords: | Osmundea pinnatifida; Sargassum muticum; enzymatic extracts; minerals; mono and polysaccharides; FTIR-ATR; NMR; cytotoxicity | Issue Date: | 3-Apr-2019 | Publisher: | MDPI | Project: | This work was supported by national funds through Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Educação (MEC) (Programa de Investimentos e Despesas de Desenvolvimento da Administração Central—PIDDAC), through project references IF/00588/2015 and IF/00407/2013/CP1162/CT0023 as well by Fundo Europeu de Desenvolvimento Regional (FEDER) through project NORTE-01-0145-FEDER-000012 (Structured Programme on Bioengineering Therapies for Infectious) funded by NORTE2020 program. This work was also supported by the Fundação para a Ciência e a Tecnologia (FCT), through the strategic project UID/MAR/04292/2019 granted to MARE. Thanks, are also due, for the financial support, to CESAM (UID/AMB/50017) through FCT/MEC national funds. | Serial title, monograph or event: | Marine Drugs | Volume: | 17 | Issue: | 4 | Abstract: | Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2-27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4-2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes. | URI: | https://hdl.handle.net/10316/107135 | ISSN: | 1660-3397 | DOI: | 10.3390/md17040209 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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