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|Title:||Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines||Authors:||Souto, Eliana B.
Souto, Selma B.
Horbańczuk, Olaf K.
Atanasov, Atanas G.
Andrade, Luciana N.
Silva, Amélia M.
|Keywords:||perillaldehyde 1,2-epoxide; Compritol ATO 888; cationic SLN; streptavidin adsorption; MCF-7 cells||Issue Date:||16-Feb-2020||Publisher:||MDPI||Project:||CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico
CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe)
|Serial title, monograph or event:||Pharmaceutics||Volume:||12||Issue:||2||Abstract:||We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer-Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN.||URI:||http://hdl.handle.net/10316/106535||ISSN:||1999-4923||DOI:||10.3390/pharmaceutics12020161||Rights:||openAccess|
|Appears in Collections:||FFUC- Artigos em Revistas Internacionais|
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