Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106517
DC Field | Value | Language |
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dc.contributor.author | Sorino, Cristina | - |
dc.contributor.author | Catena, Valeria | - |
dc.contributor.author | Bruno, Tiziana | - |
dc.contributor.author | De Nicola, Francesca | - |
dc.contributor.author | Scalera, Stefano | - |
dc.contributor.author | Bossi, Gianluca | - |
dc.contributor.author | Fabretti, Francesca | - |
dc.contributor.author | Mano, Miguel | - |
dc.contributor.author | De Smaele, Enrico | - |
dc.contributor.author | Fanciulli, Maurizio | - |
dc.contributor.author | Iezzi, Simona | - |
dc.date.accessioned | 2023-04-06T08:28:40Z | - |
dc.date.available | 2023-04-06T08:28:40Z | - |
dc.date.issued | 2020-06-19 | - |
dc.identifier.issn | 0305-1048 | pt |
dc.identifier.issn | 1362-4962 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106517 | - |
dc.description.abstract | Originally identified as an RNA polymerase II interactor, Che-1/AATF (Che-1) has now been recognized as a multifunctional protein involved in cell-cycle regulation and cancer progression, as well as apoptosis inhibition and response to stress. This protein displays a peculiar nucleolar localization and it has recently been implicated in pre-rRNA processing and ribosome biogenesis. Here, we report the identification of a novel function of Che-1 in the regulation of ribosomal RNA (rRNA) synthesis, in both cancer and normal cells. We demonstrate that Che-1 interacts with RNA polymerase I and nucleolar upstream binding factor (UBF) and promotes RNA polymerase I-dependent transcription. Furthermore, this protein binds to the rRNA gene (rDNA) promoter and modulates its epigenetic state by contrasting the recruitment of HDAC1. Che-1 downregulation affects RNA polymerase I and UBF recruitment on rDNA and leads to reducing rDNA promoter activity and 47S pre-rRNA production. Interestingly, Che-1 depletion induces abnormal nucleolar morphology associated with re-distribution of nucleolar proteins. Finally, we show that upon DNA damage Che-1 re-localizes from rDNA to TP53 gene promoter to induce cell-cycle arrest. This previously uncharacterized function of Che-1 confirms the important role of this protein in the regulation of ribosome biogenesis, cellular proliferation and response to stress. | pt |
dc.language.iso | eng | pt |
dc.publisher | Oxford University Press | pt |
dc.relation | Italian Association for Cancer Research [15255 to M.F.] | pt |
dc.relation | Funding for open access charge: Italian Ministery of Health - Ricerca Corrente 2019 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt |
dc.subject.mesh | Apoptosis Regulatory Proteins | pt |
dc.subject.mesh | Ataxia Telangiectasia Mutated Proteins | pt |
dc.subject.mesh | Cell Cycle Checkpoints | pt |
dc.subject.mesh | Cell Line | pt |
dc.subject.mesh | Cell Nucleolus | pt |
dc.subject.mesh | DNA Damage | pt |
dc.subject.mesh | DNA, Ribosomal | pt |
dc.subject.mesh | Genes, rRNA | pt |
dc.subject.mesh | Homeostasis | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Phosphorylation | pt |
dc.subject.mesh | Pol1 Transcription Initiation Complex Proteins | pt |
dc.subject.mesh | Promoter Regions, Genetic | pt |
dc.subject.mesh | RNA Polymerase I | pt |
dc.subject.mesh | Repressor Proteins | pt |
dc.subject.mesh | Ribosomes | pt |
dc.subject.mesh | Transcription, Genetic | pt |
dc.title | Che-1/AATF binds to RNA polymerase I machinery and sustains ribosomal RNA gene transcription | pt |
dc.type | article | - |
degois.publication.firstPage | 5891 | pt |
degois.publication.lastPage | 5906 | pt |
degois.publication.issue | 11 | pt |
degois.publication.title | Nucleic Acids Research | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1093/nar/gkaa344 | pt |
degois.publication.volume | 48 | pt |
dc.date.embargo | 2020-06-19 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-1922-4824 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Che-1 AATF binds to RNA polymerase I machinery.pdf | 5.79 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License