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Title: Sucupira Oil-Loaded Nanostructured Lipid Carriers (NLC): Lipid Screening, Factorial Design, Release Profile, and Cytotoxicity
Authors: Vieira, Raquel 
Severino, Patrícia 
Nalone, Luciana A.
Souto, Selma B.
Silva, Amélia M.
Lucarini, Massimo
Durazzo, Alessandra
Santini, Antonello
Souto, Eliana B. 
Keywords: essential oil; sucupira oil; diabetes mellitus; nanostructured lipid carriers (NLC); hot HPH; cytotoxicity
Issue Date: 6-Feb-2020
Publisher: MDPI
Project: M-ERA-NET/0004/2015-PAIRED, UIDB/04469/2020 (CEB strategic fund) 
PEst-OE/UID/AGR/04033/2019 (CITAB strategic fund) 
Nutraceutica come supporto nutrizionale nel paziente oncologico, CUP: B83D18000140007 
Serial title, monograph or event: Molecules
Volume: 25
Issue: 3
Abstract: Essential oils are odorant liquid oily products consisting of a complex mixture of volatile compounds obtained from a plant raw material. They have been increasingly proven to act as potential natural agents in the treatment of several human conditions, including diabetes mellitus (DM). DM is a metabolic disorder characterized by chronic hyperglycemia closely related to carbohydrate, protein and fat metabolism disturbances. In order to explore novel approaches for the management of DM our group proposes the encapsulation of sucupira essential oil, obtained from the fruits of the Brazilian plants of the genus Pterodon, in nanostructured lipid carriers (NLCs), a second generation of lipid nanoparticles which act as new controlled drug delivery system (DDS). Encapsulation was performed by hot high-pressure homogenization (HPH) technique and the samples were then analyzed by dynamic light scattering (DLS) for mean average size and polydispersity index (PI) and by electrophoretic light scattering (ELS) for zeta potential (ZP), immediately after production and after 24 h of storage at 4 °C. An optimal sucupira-loaded NLC was found to consist of 0.5% (m/V) sucupira oil, 4.5% (m/V) of Kollivax® GMS II and 1.425% (m/V) of TPGS (formulation no. 6) characterized by a mean particle size ranging from 148.1 ± 0.9815 nm (0 h) to 159.3 ± 9.539 nm (at 24 h), a PI from 0.274 ± 0.029 (0 h) to 0.305 ± 0.028 (24 h) and a ZP from -0.00236 ± 0.147 mV (at 0 h) to 0.125 ± 0.162 (at 24 h). The encapsulation efficiency and loading capacity were 99.98% and 9.6%, respectively. The optimized formulation followed a modified release profile fitting the first order kinetics, over a period of 8 h. In vitro cytotoxicity studies were performed against Caco-2 cell lines, for which the cell viability above 90% confirmed the non-cytotoxic profile of both blank and sucupira oil-loaded NLC.
ISSN: 1420-3049
DOI: 10.3390/molecules25030685
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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