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Title: Complex Polysaccharide-Based Nanocomposites for Oral Insulin Delivery
Authors: Collado-González, Mar
Ferreri, M. Cristina
Freitas, Alessandra
Santos, Ana Cláudia 
Ferreira, Nuno Ricardo Esteves 
Carissimi, Guzmán
Sequeira, Joana A. D. 
Díaz Baños, F. Guillermo
Villora, Gloria
Veiga, Francisco 
Ribeiro, António 
Keywords: chitosan; alginate polysaccharide nanocomposite; stability; LUMiSizer
Issue Date: 15-Jan-2020
Publisher: MDPI
Serial title, monograph or event: Marine Drugs
Volume: 18
Issue: 1
Abstract: Polyelectrolyte nanocomposites rarely reach a stable state and aggregation often occurs. Here, we report the synthesis of nanocomposites for the oral delivery of insulin composed of alginate, dextran sulfate, poly-(ethylene glycol) 4000, poloxamer 188, chitosan, and bovine serum albumin. The nanocomposites were obtained by Ca2+-induced gelation of alginate followed by an electrostatic-interaction process among the polyelectrolytes. Chitosan seemed to be essential for the final size of the nanocomposites and there was an optimal content that led to the synthesis of nanocomposites of 400-600 nm hydrodynamic size. The enhanced stability of the synthesized nanocomposites was assessed with LUMiSizer after synthesis. Nanocomposite stability over time and under variations of ionic strength and pH were assessed with dynamic light scattering. The rounded shapes of nanocomposites were confirmed by scanning electron microscopy. After loading with insulin, analysis by HPLC revealed complete drug release under physiologically simulated conditions.
ISSN: 1660-3397
DOI: 10.3390/md18010055
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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