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Title: Hepatic Glycerol Metabolism-Related Genes in Carnivorous Rainbow Trout (Oncorhynchus mykiss): Insights Into Molecular Characteristics, Ontogenesis, and Nutritional Regulation
Authors: Panserat, Stephane
Plagnes-Juan, Elisabeth
Gazzola, Elsa
Palma, Mariana 
Magnoni, Leonardo J.
Marandel, Lucie
Viegas, Ivan 
Keywords: glycerol metabolism; fish nutrition; genomics; gene expression; rainbow trout; aquaculture
Issue Date: 2020
Publisher: Frontiers Media S.A.
Serial title, monograph or event: Frontiers in Physiology
Volume: 11
Abstract: Glycerol metabolism in rainbow trout is poorly studied even though it is at the interface between lipid and glucose metabolism. Moreover, glycerol can be an important ingredient in new aquafeed formulation to decrease the catabolism of dietary amino acids. Thus, the present study aimed to characterize for the first time the different genes coding for key enzymes and proteins involved in hepatic glycerol metabolism. From the trout genomes, all the paralogous genes coding for glycerol transport (aqp9b), glycerol kinase (gk2a and gk5), glycerol-3-phosphate phosphatase (pgp), and glycerol-3-phosphate dehydrogenase (gpd1a, gpd1b, and gpd1c) were identified. The ontogenesis determined that the capacity to metabolize glycerol begins with the apparition of the liver during the development (stage 22) and are more expressed at the endogenous-exogenous feeding period (stage 35). The postprandial regulation of the expression of these genes in juvenile trout showed that the postprandial peak of expression is between 4 and 24 h after the last meal for many of the genes, demonstrating that glycerol metabolism could be nutritionally regulated at a molecular level. However, surprisingly, no regulation of the mRNA abundance for the glycerol metabolism-related genes by different levels of dietary glycerol (0, 2.5, and 5%) have been detected, showing that hepatic glycerol metabolism is poorly regulated at a molecular level by dietary glycerol in rainbow trout juveniles.
ISSN: 1664-042X
DOI: 10.3389/fphys.2020.00882
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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