Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106112
DC Field | Value | Language |
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dc.contributor.author | Maldonado-Carmona, Nidia | - |
dc.contributor.author | Marchand, Guillaume | - |
dc.contributor.author | Villandier, Nicolas | - |
dc.contributor.author | Ouk, Tan-Sothea | - |
dc.contributor.author | Pereira, Mariette M. | - |
dc.contributor.author | Calvete, Mário J. F. | - |
dc.contributor.author | Calliste, Claude Alain | - |
dc.contributor.author | Żak, Andrzej | - |
dc.contributor.author | Piksa, Marta | - |
dc.contributor.author | Pawlik, Krzysztof J | - |
dc.contributor.author | Matczyszyn, Katarzyna | - |
dc.contributor.author | Leroy-Lhez, Stéphanie | - |
dc.date.accessioned | 2023-03-21T10:15:57Z | - |
dc.date.available | 2023-03-21T10:15:57Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1664-302X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106112 | - |
dc.description.abstract | The need for alternative strategies to fight bacteria is evident from the emergence of antimicrobial resistance. To that respect, photodynamic antimicrobial chemotherapy steadily rises in bacterial eradication by using light, a photosensitizer and oxygen, which generates reactive oxygen species that may kill bacteria. Herein, we report the encapsulation of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphyrin into acetylated lignin water-dispersible nanoparticles (THPP@AcLi), with characterization of those systems by standard spectroscopic and microscopic techniques. We observed that THPP@AcLi retained porphyrin's photophysical/photochemical properties, including singlet oxygen generation and fluorescence. Besides, the nanoparticles demonstrated enhanced stability on storage and light bleaching. THPP@AcLi were evaluated as photosensitizers against two Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, and against three Gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. THPP@AcLi were able to diminish Gram-positive bacterial survival to 0.1% when exposed to low white LED light doses (4.16 J/cm2), requiring concentrations below 5 μM. Nevertheless, the obtained nanoparticles were unable to diminish the survival of Gram-negative bacteria. Through transmission electron microscopy observations, we could demonstrate that nanoparticles did not penetrate inside the bacterial cell, exerting their destructive effect on the bacterial wall; also, a high affinity between acetylated lignin nanoparticles and bacteria was observed, leading to bacterial flocculation. Altogether, these findings allow to establish a photodynamic antimicrobial chemotherapy alternative that can be used effectively against Gram-positive topic infections using the widely available natural polymeric lignin as a drug carrier. Further research, aimed to inhibit the growth and survival of Gram-negative bacteria, is likely to enhance the wideness of acetylated lignin nanoparticle applications. | pt |
dc.language.iso | eng | pt |
dc.publisher | Frontiers Media S.A. | pt |
dc.relation | Marie Skłodowska-Curie grant agreement no. 764837 | pt |
dc.relation | National Science Centre (PL) under “Miniatura” grant no. 2019/03/X/NZ3/02100 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | tetrapyrrolic compounds | pt |
dc.subject | valorized lignin | pt |
dc.subject | nanoparticle | pt |
dc.subject | photodynamic antimicrobial therapy | pt |
dc.subject | antimicrobial alternatives | pt |
dc.title | Porphyrin-Loaded Lignin Nanoparticles Against Bacteria: A Photodynamic Antimicrobial Chemotherapy Application | pt |
dc.type | article | - |
degois.publication.firstPage | 606185 | pt |
degois.publication.title | Frontiers in Microbiology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3389/fmicb.2020.606185 | pt |
degois.publication.volume | 11 | pt |
dc.date.embargo | 2020-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0003-4958-7677 | - |
crisitem.author.orcid | 0000-0003-2094-4781 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Porphyrin-Loaded Lignin Nanoparticles Against Bacteria A Photodynamic Antimicrobial Chemotherapy Application.pdf | 18.42 MB | Adobe PDF | View/Open |
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