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Title: Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses
Authors: Pandian, Swarna
Zhao, Jian-Ping
Murata, Yasunobu
Bustos, Fernando J
Tunca, Cansu
Almeida, Ramiro D. 
Constantine-Paton, Martha
Keywords: anxiety; autism spectrum disorder; LTD; transport
Issue Date: 2020
Publisher: Society for Neuroscience
Project: National Institutes of Health Grants R01- EY014074 and R01-EY014420 
MIT Portugal Program, the Portuguese Foundation for Science and Technology Grant SFRH/BD/33726/2009 
McGovern Institute for Brain Research 
Simons Center for the Social Brain 
FONDECYT (Fondo Nacional de Desarrollo Científico y Tecnológico) 11180540 
ANID (Agencia Nacional de Investigación y Desarrollo de Chile) PAI 77180077 
Serial title, monograph or event: eNeuro
Volume: 7
Issue: 6
Abstract: Myosin Va (MyoVa) is a plus-end filamentous-actin motor protein that is highly and broadly expressed in the vertebrate body, including in the nervous system. In excitatory neurons, MyoVa transports cargo toward the tip of the dendritic spine, where the postsynaptic density (PSD) is formed and maintained. MyoVa mutations in humans cause neurologic dysfunction, intellectual disability, hypomelanation, and death in infancy or childhood. Here, we characterize the Flailer (Flr) mutant mouse, which is homozygous for a myo5a mutation that drives high levels of mutant MyoVa (Flr protein) specifically in the CNS. Flr protein functions as a dominant-negative MyoVa, sequestering cargo and blocking its transport to the PSD. Flr mice have early seizures and mild ataxia but mature and breed normally. Flr mice display several abnormal behaviors known to be associated with brain regions that show high expression of Flr protein. Flr mice are defective in the transport of synaptic components to the PSD and in mGluR-dependent long-term depression (LTD) and have a reduced number of mature dendritic spines. The synaptic and behavioral abnormalities of Flr mice result in anxiety and memory deficits similar to that of other mouse mutants with obsessive-compulsive disorder and autism spectrum disorder (ASD). Because of the dominant-negative nature of the Flr protein, the Flr mouse offers a powerful system for the analysis of how the disruption of synaptic transport and lack of LTD can alter synaptic function, development and wiring of the brain and result in symptoms that characterize many neuropsychiatric disorders.
ISSN: 2373-2822
DOI: 10.1523/ENEURO.0284-20.2020
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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