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Title: Heat Stress in Pinus halepensis Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
Authors: Pereira, Cátia 
Castander-Olarieta, Ander
Sales, Ester
Montalbán, Itziar A
Canhoto, Jorge 
Moncaleán, Paloma
Keywords: Aleppo pine; conifers; DEHYDRATION INDUCED PROTEIN 19; epigenetics; priming; SUPEROXIDE DISMUTASE [Cu–Zn]; 5-hydroxymethylcytosine; 5-methylcytosine
Issue Date: 29-Oct-2021
Project: MINECO (Spanish Government) project (AGL2016-76143- C4-3R), BIOALI-CYTED (P117RT0522) 
DECO (Basque government, Ayudas de formación a jóvenes investigadores y tecnólogos) 
F4F-Forest for the future (CENTRO-08-5864-FSE-000031 
ERA-NET cofund Forest Value by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE)) 
Forest Value has received funding from the European Union’s Horizon 2020 research and innovation programmed under agreement No 773324 
Serial title, monograph or event: Plants
Volume: 10
Issue: 11
Abstract: In the current context of climate change, plants need to develop different mechanisms of stress tolerance and adaptation to cope with changing environmental conditions. Temperature is one of the most important abiotic stresses that forest trees have to overcome. Recent research developed in our laboratory demonstrated that high temperatures during different stages of conifer somatic embryogenesis (SE) modify subsequent phases of the process and the behavior of the resulting ex vitro somatic plants. For this reason, Aleppo pine SE was induced under different heat stress treatments (40 °C for 4 h, 50 °C for 30 min, and 60 °C for 5 min) in order to analyze its effect on the global DNA methylation rates and the differential expression of four stress-related genes at different stages of the SE process. Results showed that a slight decrease of DNA methylation at proliferating embryonal masses (EMs) can correlate with the final efficiency of the process. Additionally, different expression patterns for stress-related genes were found in EMs and needles from the in vitro somatic plants obtained; the DEHYDRATION INDUCED PROTEIN 19 gene was up-regulated in response to heat at proliferating EMs, whereas HSP20 FAMILY PROTEIN and SUPEROXIDE DISMUTASE [Cu-Zn] were down-regulated in needles.
ISSN: 2223-7747
DOI: 10.3390/plants10112333
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais

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