Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/105269
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Alves, Celso | - |
dc.contributor.author | Silva, Joana | - |
dc.contributor.author | Pinteus, Susete | - |
dc.contributor.author | Alonso, Eva | - |
dc.contributor.author | Alvariño, Rebeca | - |
dc.contributor.author | Duarte, Adriana | - |
dc.contributor.author | Marmitt, Diorge | - |
dc.contributor.author | Goettert, Márcia Inês | - |
dc.contributor.author | Gaspar, Helena | - |
dc.contributor.author | Alfonso, Amparo | - |
dc.contributor.author | Alpoim, Maria C. | - |
dc.contributor.author | Botana, Luis M. | - |
dc.contributor.author | Pedrosa, Rui | - |
dc.date.accessioned | 2023-02-13T12:41:15Z | - |
dc.date.available | 2023-02-13T12:41:15Z | - |
dc.date.issued | 2021-03-04 | - |
dc.identifier.issn | 1420-3049 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/105269 | - |
dc.description.abstract | Marine natural products have exhibited uncommon chemical structures with relevant antitumor properties highlighting their potential to inspire the development of new anticancer agents. The goal of this work was to study the antitumor activities of the brominated diterpene sphaerodactylomelol, a rare example of the dactylomelane family. Cytotoxicity (10-100 µM; 24 h) was evaluated on tumor cells (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, SK-ML-28) and the effects estimated by MTT assay. Hydrogen peroxide (H2O2) levels and apoptosis biomarkers (membrane translocation of phosphatidylserine, depolarization of mitochondrial membrane potential, Caspase-9 activity, and DNA condensation and/or fragmentation) were studied in the breast adenocarcinoma cellular model (MCF-7) and its genotoxicity on mouse fibroblasts (L929). Sphaerodactylomelol displayed an IC50 range between 33.04 and 89.41 µM without selective activity for a specific tumor tissue. The cells' viability decrease was accompanied by an increase on H2O2 production, a depolarization of mitochondrial membrane potential and an increase of Caspase-9 activity and DNA fragmentation. However, the DNA damage studies in L929 non-malignant cell line suggested that this compound is not genotoxic for normal fibroblasts. Overall, the results suggest that the cytotoxicity of sphaerodactylomelol seems to be mediated by an increase of H2O2 levels and downstream apoptosis. | pt |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the strategic project UID/MAR/04292/2020 to MARE—Marine and Environmental Sciences Centre and UIDP/Multi/04046/2020 and UIDB/04046/2020 granted to BioISI—BioSystems and Integrative Sciences Institute, through POINT4PAC project (Oncologia de Precisão: Terapias e Tecnologias Inovadoras (SAICTPAC/0019/ 2015-LISBOA- 01-0145-FEDER-016405), through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER-029250). FCT is also acknowledged for the grant attributed to J.S. (SFRH/BD/103255/2014). | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | UID/MAR/04292/2020 | pt |
dc.relation | UIDB/04046/2020 | pt |
dc.relation | PTDC/BIA-OUT/29250/2017 | pt |
dc.relation | POCI-01-0145-FEDER-029250 | pt |
dc.relation | SFRH/BD/103255/2014 | pt |
dc.relation | UIDP/04046/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | breast cancer | pt |
dc.subject | red algae | pt |
dc.subject | oxidative stress | pt |
dc.subject | marine natural products | pt |
dc.subject | apoptosis | pt |
dc.subject | DNA damage | pt |
dc.subject | biological activities | pt |
dc.subject | MCF-7 cells | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Antineoplastic Agents | pt |
dc.subject.mesh | Breast Neoplasms | pt |
dc.subject.mesh | Cell Proliferation | pt |
dc.subject.mesh | Cells, Cultured | pt |
dc.subject.mesh | DNA Damage | pt |
dc.subject.mesh | Diterpenes | pt |
dc.subject.mesh | Female | pt |
dc.subject.mesh | Fibroblasts | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Hydrogen Peroxide | pt |
dc.subject.mesh | Membrane Potential, Mitochondrial | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Rhodophyta | pt |
dc.subject.mesh | Apoptosis | pt |
dc.title | Cytotoxic Mechanism of Sphaerodactylomelol, an Uncommon Bromoditerpene Isolated from Sphaerococcus coronopifolius | pt |
dc.type | article | - |
degois.publication.firstPage | 1374 | pt |
degois.publication.issue | 5 | pt |
degois.publication.title | Molecules | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/molecules26051374 | pt |
degois.publication.volume | 26 | pt |
dc.date.embargo | 2021-03-04 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | MARE-Marine and Environmental Sciences Centre | - |
crisitem.project.grantno | Biosystems and Integrative Sciences Institute | - |
crisitem.project.grantno | Biosystems and Integrative Sciences Institute | - |
crisitem.author.orcid | 0000-0001-9776-5701 | - |
crisitem.author.orcid | 0000-0002-1613-7023 | - |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Cytotoxic-mechanism-of-sphaerodactylomelol-an-uncommon-bromoditerpene-isolated-from-Sphaerococcus-coronopifoliusMolecules.pdf | 1.44 MB | Adobe PDF | Ver/Abrir |
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