Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/104566
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Castander-Olarieta, Ander | - |
dc.contributor.author | Pereira, Cátia | - |
dc.contributor.author | Montalbán, Itziar A | - |
dc.contributor.author | Mendes, Vera M. | - |
dc.contributor.author | Correia, Sandra | - |
dc.contributor.author | Suárez-Álvarez, Sonia | - |
dc.contributor.author | Manadas, Bruno | - |
dc.contributor.author | Canhoto, Jorge | - |
dc.contributor.author | Moncaleán, Paloma | - |
dc.date.accessioned | 2023-01-18T10:14:23Z | - |
dc.date.available | 2023-01-18T10:14:23Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1664-462X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/104566 | - |
dc.description.abstract | Somatic embryogenesis is the process by which bipolar structures with no vascular connection with the surrounding tissue are formed from a single or a group of vegetative cells, and in conifers it can be divided into five different steps: initiation, proliferation, maturation, germination and acclimatization. Somatic embryogenesis has long been used as a model to study the mechanisms regulating stress response in plants, and recent research carried out in our laboratory has demonstrated that high temperatures during initial stages of conifer somatic embryogenesis modify subsequent phases of the process, as well as the behavior of the resulting plants ex vitro. The development of high-throughput techniques has facilitated the study of the molecular response of plants to numerous stress factors. Proteomics offers a reliable image of the cell status and is known to be extremely susceptible to environmental changes. In this study, the proteome of radiata pine somatic embryos was analyzed by LC-MS after the application of high temperatures during initiation of embryonal masses [(23°C, control; 40°C (4 h); 60°C (5 min)]. At the same time, the content of specific soluble sugars and sugar alcohols was analyzed by HPLC. Results confirmed a significant decrease in the initiation rate of embryonal masses under 40°C treatments (from 44 to 30.5%) and an increasing tendency in the production of somatic embryos (from 121.87 to 170.83 somatic embryos per gram of embryogenic tissue). Besides, heat provoked a long-term readjustment of the protein synthesis machinery: a great number of structural constituents of ribosomes were increased under high temperatures, together with the down-regulation of the enzyme methionine-tRNA ligase. Heat led to higher contents of heat shock proteins and chaperones, transmembrane transport proteins, proteins related with post-transcriptional regulation (ARGONAUTE 1D) and enzymes involved in the synthesis of fatty acids, specific compatible sugars (myo-inositol) and cell-wall carbohydrates. On the other hand, the protein adenosylhomocysteinase and enzymes linked with the glycolytic pathway, nitrogen assimilation and oxidative stress response were found at lower levels. | pt |
dc.language.iso | eng | pt |
dc.publisher | Frontiers Media S.A. | pt |
dc.relation | MINECO project (AGL2016-76143- C4-3R), CYTED (P117RT0522), DECO (Basque government) and MULTIFOREVER project, supported under the umbrella of ERA-NET Cofund ForestValue by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), and VINNOVA (SE). | pt |
dc.relation | ForestValue has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement no. 773324 | pt |
dc.relation | Centro-01- 0145-FEDER-000007 | pt |
dc.relation | SFRH/BD/123702/2016) | pt |
dc.relation | POCI-01- 0145-FEDER-031999 | pt |
dc.relation | POCI-01-0145-FEDER-007440 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | POCI-01-0145-FEDER-402-022125 (ROTEIRO/0028/2013) | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | carbohydrates | pt |
dc.subject | compatible solutes | pt |
dc.subject | heat shock proteins | pt |
dc.subject | high temperatures | pt |
dc.subject | methylation | pt |
dc.subject | proteomics | pt |
dc.subject | radiata pine | pt |
dc.subject | somatic embryogenesis | pt |
dc.title | Proteome-Wide Analysis of Heat-Stress in Pinus radiata Somatic Embryos Reveals a Combined Response of Sugar Metabolism and Translational Regulation Mechanisms | pt |
dc.type | article | - |
degois.publication.firstPage | 631239 | pt |
degois.publication.title | Frontiers in Plant Science | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3389/fpls.2021.631239 | pt |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2021-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.orcid | 0000-0002-1033-8270 | - |
crisitem.author.orcid | 0000-0002-4593-673X | - |
crisitem.author.orcid | 0000-0003-2151-3916 | - |
crisitem.author.orcid | 0000-0002-2087-4042 | - |
crisitem.author.orcid | 0000-0003-2299-298X | - |
Appears in Collections: | I&D CFE - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
fpls-12-631239.pdf | 1.53 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
14
checked on Aug 19, 2024
WEB OF SCIENCETM
Citations
13
checked on Aug 2, 2024
Page view(s)
82
checked on Aug 27, 2024
Download(s)
47
checked on Aug 27, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License