Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/103330
Title: PEG35 as a Preconditioning Agent against Hypoxia/Reoxygenation Injury
Authors: Silva, Rui Teixeira da 
Machado, Ivo F. 
Teodoro, João S. 
Panisello-Roselló, Arnau
Roselló-Catafau, Joan 
Rolo, Anabela P. 
Palmeira, Carlos M. 
Keywords: polyethylene glycol 35; hypoxia/reoxygenation injury; mitochondria; autophagy
Issue Date: 21-Jan-2022
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 23
Issue: 3
Abstract: Pharmacological conditioning is a protective strategy against ischemia/reperfusion injury, which occurs during liver resection and transplantation. Polyethylene glycols have shown multiple benefits in cell and organ preservation, including antioxidant capacity, edema prevention and membrane stabilization. Recently, polyethylene glycol 35 kDa (PEG35) preconditioning resulted in decreased hepatic injury and protected the mitochondria in a rat model of cold ischemia. Thus, the study aimed to decipher the mechanisms underlying PEG35 preconditioning-induced protection against ischemia/reperfusion injury. A hypoxia/reoxygenation model using HepG2 cells was established to evaluate the effects of PEG35 preconditioning. Several parameters were assessed, including cell viability, mitochondrial membrane potential, ROS production, ATP levels, protein content and gene expression to investigate autophagy, mitochondrial biogenesis and dynamics. PEG35 preconditioning preserved the mitochondrial function by decreasing the excessive production of ROS and subsequent ATP depletion, as well as by recovering the membrane potential. Furthermore, PEG35 increased levels of autophagy-related proteins and the expression of genes involved in mitochondrial biogenesis and fusion. In conclusion, PEG35 preconditioning effectively ameliorates hepatic hypoxia/reoxygenation injury through the enhancement of autophagy and mitochondrial quality control. Therefore, PEG35 could be useful as a potential pharmacological tool for attenuating hepatic ischemia/reperfusion injury in clinical practice.
URI: https://hdl.handle.net/10316/103330
ISSN: 1422-0067
DOI: 10.3390/ijms23031156
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais

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