Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/103315
Title: Cold Atmospheric Plasma Apoptotic and Oxidative Effects on MCF7 and HCC1806 Human Breast Cancer Cells
Authors: Almeida-Ferreira, Catarina 
Silva-Teixeira, Rafael 
Gonçalves, Ana Cristina 
Marto, Carlos Miguel 
Sarmento-Ribeiro, Ana Bela 
Caramelo, Francisco 
Botelho, Maria Filomena 
Laranjo, Mafalda 
Keywords: breast neoplasms; cold atmospheric plasma; plasma gases; reactive nitrogen species; reactive oxygen species
Issue Date: 1-Feb-2022
Project: UID/NEU/04539/2019 
UIDB/04539/2020 
UIDP/04539/2020 
POCI-01-0145-FEDER-007440 
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 23
Issue: 3
Abstract: Breast cancer (BC) is a malignant neoplasia with the highest incidence and mortality rates in women worldwide. Currently, therapies include surgery, radiotherapy, and chemotherapy, including targeted therapies in some cases. However, treatments are often associated with serious adverse effects. Looking for new options in BC treatment, we evaluated the therapeutic potential of cold atmospheric plasma (CAP) in two cell lines (MCF7 and HCC1806) with distinct histological features. Apoptosis seemed to be the most prevalent type of death, as corroborated by several biochemical features, including phosphatidylserine exposure, the disruption of mitochondrial membrane potential, an increase in BAX/BCL2 ratio and procaspase 3 loss. Moreover, the accumulation of cells in the G2/M phase of the cell cycle points to the loss of replication ability and decreased survival. Despite reported toxic concentrations of peroxides in culture media exposed to plasma, intracellular peroxide concentration was overall decreased accompanying a reduction in GSH levels shortly after plasma exposure in both cell lines. In HCC1806, elevated nitric oxide (NO) concentration accompanied by reduced superoxide levels suggests that these cells are capable of converting plasma-derived nitrites into NO that competes with superoxide dismutase (SOD) for superoxide to form peroxinitrite. The concomitant inhibition of the antioxidative activity of cells during CAP treatment, particularly the inhibition of cytochrome c oxidase with sodium azide, synergistically increased plasma toxicity. Thus, this in vitro research enlightens the therapeutic potential of CAP in the treatment of breast cancer, elucidating its possible mechanisms of action.
URI: https://hdl.handle.net/10316/103315
ISSN: 1422-0067
DOI: 10.3390/ijms23031698
Rights: openAccess
Appears in Collections:I&D ICBR - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais

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