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Title: Differential immuno-reactivity to genomic DNA, RNA and mitochondrial DNA is associated with auto-immunity
Authors: Ivanova, Vilena V 
Khaiboullina, Svetlana F
Cherenkova, Ekaterina E
Martynova, Ekaterina V
Nevzorova, Tatiana A
Kunst, Michail A
Sibgatullin, Timur B
Maksudova, Adelia N
Oliveira, Paulo J. 
Lombardi, Vincent C
Palotas, Andras 
Rizvanov, Albert A 
Keywords: Auto-antibody; Auto-antigens; Auto-immune disease; Biomarker; Mitochondri; Nucleic acid; Systemic lupus erythematosus (SLE)
Issue Date: 2014
Project: Russian Government Program of Competitive Growth of Kazan Federal University and funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities. 
Serial title, monograph or event: Cellular Physiology and Biochemistry
Volume: 34
Issue: 6
Abstract: Circulating auto-reactive antibodies are hallmark features of auto-immune diseases, however little is known with respect to the specificity of such bio-markers. In the present study, we investigated the specificity of anti-nucleic acid antibodies in the blood of subjects with systemic lupus erythematosus (SLE) and healthy controls. Methods: Sera from 12 SLE cases and 8 controls were evaluated for immuno-reactivity to purified RNA, DNA and mitochondrial DNA (mtDNA) by enzyme-linked immuno-sorbent assay (ELISA). Results: As expected, immuno-reactivity to total nucleic acids was significantly higher in subjects with SLE when compared to healthy controls, however a clear distinction was observed among the various nucleic acid sub-types, with sera from SLE subjects displaying the greatest immunoreactivity to RNA followed by mtDNA and then total DNA. Conclusion: The identification of auto-reactive antibodies can serve as highly sensitive biomarkers, although their specificity may not always allow diagnostic certainty. The knowledge that auto-antibodies in subjects with SLE display differential immuno-reactivity may help to improve existing diagnostics and may lead to a better understanding of the pathogenesis of auto-immune disorders.
ISSN: 1421-9778
DOI: 10.1159/000369663
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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