Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/100785
DC FieldValueLanguage
dc.contributor.authorCarvalhais, Lia G.-
dc.contributor.authorMartinho, Vera C.-
dc.contributor.authorFerreiro, Elisabete-
dc.contributor.authorPinheiro, Paulo S.-
dc.date.accessioned2022-07-11T10:02:25Z-
dc.date.available2022-07-11T10:02:25Z-
dc.date.issued2020-
dc.identifier.issn1662-4548pt
dc.identifier.urihttps://hdl.handle.net/10316/100785-
dc.description.abstractThe complex, nanoscopic scale of neuronal function, taking place at dendritic spines, axon terminals, and other minuscule structures, cannot be adequately resolved using standard, diffraction-limited imaging techniques. The last couple of decades saw a rapid evolution of imaging methods that overcome the diffraction limit imposed by Abbe's principle. These techniques, including structured illumination microscopy (SIM), stimulated emission depletion (STED), photo-activated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM), among others, have revolutionized our understanding of synapse biology. By exploiting the stochastic nature of fluorophore light/dark states or non-linearities in the interaction of fluorophores with light, by using modified illumination strategies that limit the excitation area, these methods can achieve spatial resolutions down to just a few tens of nm or less. Here, we review how these advanced imaging techniques have contributed to unprecedented insight into the nanoscopic organization and function of mammalian neuronal presynapses, revealing new organizational principles or lending support to existing views, while raising many important new questions. We further discuss recent technical refinements and newly developed tools that will continue to expand our ability to delve deeper into how synaptic function is orchestrated at the nanoscopic level.pt
dc.language.isoengpt
dc.relationproject CENTRO-01-0145-FEDER-000008pt
dc.relationBrainHealth 2020 (scholarship to VM)pt
dc.relationUIDB/04539/2020pt
dc.relationgrant PTDC/BIA-CEL/29451/2017pt
dc.relationgrant CEECIND/00003/2018pt
dc.relationgrant CEECIND/00322/2017pt
dc.relationFCT “Summer with Science” research fellowshippt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectactive zonept
dc.subjectneurotransmitter releasept
dc.subjectpresynaptic structurept
dc.subjectsuper-resolution microscopypt
dc.subjectvesicle exocytosispt
dc.titleUnraveling the Nanoscopic Organization and Function of Central Mammalian Presynapses With Super-Resolution Microscopypt
dc.typearticle-
degois.publication.firstPage578409pt
degois.publication.titleFrontiers in Neurosciencept
dc.peerreviewedyespt
dc.identifier.doi10.3389/fnins.2020.578409pt
degois.publication.volume14pt
dc.date.embargo2020-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypearticle-
crisitem.author.orcid0000-0003-2689-2396-
crisitem.author.orcid0000-0002-3953-3088-
crisitem.author.orcid0000-0002-1200-4602-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
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