Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/100575
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dc.contributor.authorAbreu, Ricardo C de-
dc.contributor.authorRamos, Cristiana V-
dc.contributor.authorBecher, Clarissa-
dc.contributor.authorLino, Miguel-
dc.contributor.authorJesus, Carlos-
dc.contributor.authorMartins, Paula A da Costa-
dc.contributor.authorMartins, Patrícia A T-
dc.contributor.authorMoreno, Maria João-
dc.contributor.authorFernandes, Hugo-
dc.contributor.authorFerreira, Lino-
dc.date.accessioned2022-07-03T16:32:04Z-
dc.date.available2022-07-03T16:32:04Z-
dc.date.issued2021-08-
dc.identifier.issn2001-3078pt
dc.identifier.urihttps://hdl.handle.net/10316/100575-
dc.description.abstractSmall extracellular vesicles (sEVs), through their natural ability to interact with biological membranes and exploit endogenous processing pathways to convey biological information, are quintessential for the delivery of therapeutically relevant compounds, such as microRNAs (miRNAs) and proteins. Here, we used a fluorescently-labelled miRNA to quantify the efficiency of different methods to modulate the cargo of sEVs. Our results showed that, compared with electroporation, heat shock, permeation by a detergent-based compound (saponin) or cholesterol-modification of the miRNA, Exo-Fect was the most efficient method with > 50% transfection efficiency. Furthermore, qRT-PCR data showed that, compared with native sEVs, Exo-Fect modulation led to a > 1000-fold upregulation of the miRNA of interest. Importantly, this upregulation was observed for sEVs isolated from multiple sources. The modulated sEVs were able to delivery miR-155-5p into a reporter cell line, confirming the successful delivery of the miRNA to the target cell and, more importantly, its functionality. Finally, we showed that the membrane of Exo-Fect-loaded sEVs was altered compared with native sEVs and that enhanced the internalization of Exo-Fect-loaded sEVs within the target cells and decreased the interaction of those modulated sEVs with lysosomes.pt
dc.language.isoengpt
dc.relation007630 UID/QUI/00313/2019pt
dc.relationPT2020_PTDC_DTP-FTO_2784_2014pt
dc.relationPOCI-01-0145-FEDER-029919pt
dc.relationCOMPETE2020- UEpt
dc.relationCENTRO-01-0145-FEDER-000014pt
dc.relationPrograma Operacional Regional do Centro’ CENTRO2020pt
dc.relationProjects Interreg entitled: ‘Impulso de una red de I+i en química biológica para diagnóstico y tratamiento de enfermedades neurológicaspt
dc.relationEAPA_791/2018 - NEUROATLANTIC entitled: ‘An Atlantic innovation platform on diagnosis and treatment of neurological diseases and aging’pt
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/952266/EU/RESEarch for healThy AGEINGpt
dc.rightsopenAccesspt
dc.subjectextracellular vesicles; microRNA; modulation; post‐isolationpt
dc.subject.meshCell Linept
dc.subject.meshDrug Delivery Systemspt
dc.subject.meshExtracellular Vesiclespt
dc.subject.meshGenetic Vectorspt
dc.subject.meshHEK293 Cellspt
dc.subject.meshHumanspt
dc.subject.meshMicroRNAspt
dc.subject.meshMicroscopy, Electron, Transmissionpt
dc.subject.meshGene Transfer Techniquespt
dc.titleExogenous loading of miRNAs into small extracellular vesiclespt
dc.typearticle-
degois.publication.firstPagee12111pt
degois.publication.issue10pt
degois.publication.titleJ Extracell Vesiclespt
dc.peerreviewedyespt
dc.identifier.doi10.1002/jev2.12111pt
degois.publication.volume10pt
dc.date.embargo2021-08-01*
uc.date.periodoEmbargo0pt
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.cerifentitytypePublications-
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais
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