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Title: Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry
Authors: Mariz-Ponte, Nuno
Sario, Sara
Mendes, Rafael J. 
Couto, Márcio
Gimranov, Emil
Santos, Marino
Correia, Cristiana V.
Gomes, Anicia
Oliveira-Pinto, Paulo R.
Amorim, Isabel 
Dias, Maria Celeste 
Oliveira, José Miguel P. Ferreira de
Santos, Conceição
Keywords: bioactivity; crop growth; gene regulation; photosynthesis; titanium-dioxide nanoparticles
Issue Date: 2022
Project: UID/QUI/50006/2020 
FCT grant number SFRH/BPD/74868/2010 
DL 57/2016-Norma transitória 
FCT grant number SFRH/BPD/100865/2014 
Serial title, monograph or event: Agriculture (Switzerland)
Volume: 12
Issue: 2
Abstract: Industrial applications of titanium dioxide nanoparticles (TiO2 NPs) are wide, and their use in nano-fertilizing technology has been proposed in the last few years. Bioactivity evaluation of different TiO2 NPs formulations is therefore crucial, not only to select the most appropriate formulation but also to validate potential agro-applications. In the current work, we compared the bioactivity of the two most used TiO2 NPs formulations (anatase and rutile–anatase) on the photosynthesis of Lactuca sativa. Seeds were exposed to concentrations of 0, 10, and 50 mg L−1 of anatase (A) or rutile–anatase (RA). Germination rate was not affected by NPs, but root growth was stimulated mainly by RA50. Compared with control, RA showed positive effects on photophosphorylation-related parameters. A50 was more efficient in promoting the gas exchange phase (PN, Ci, gs, and E) and in stimulating the absorption of some nutrients. Expanding on the biochemical and physiological data, we show that RA50 stimulated several genes coding for proteins involved in the electron transport in thylakoids (psbA, petB, petA, psaA, psaC, ndhA, ndhD) and ATP synthesis (atpA, atpB). The transcript coding for the large subunit of RuBisCO (rbcL), was stimulated by lower concentration (RA10). This suggests that RuBisCO is highly sensitive to these NPs even at low doses. RA at low doses has been demonstrated to be the most promising NP. These discriminative effects of TiO2 NPs, based on their formulation and dose, may present advantages for their use in the precision nanoagroindustry.
ISSN: 2077-0472
DOI: 10.3390/agriculture12020190
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais

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