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Title: | Exploring the antioxidant, anti-inflammatory and antiallergic potential of Brazilian propolis in monocytes | Authors: | Conte, Fernanda Lopes Pereira, Ana Catarina Brites, Gonçalo Ferreira, Isabel Silva, Ana Cristina Sebastião, Ana Isabel Matos, Patrícia Pereira, Cláudia Batista, Maria Teresa Sforcin, José Maurício Cruz, Maria Teresa |
Keywords: | Anti-inflammatory action; Antiallergic action; Antioxidant activity; Monocyte; Propolis | Issue Date: | 2022 | Project: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/Programa Institucional de Internacionalização (CAPES/PRINT) under grant number: 88887.467268/2019–00 FCT |
Serial title, monograph or event: | Phytomedicine Plus | Volume: | 2 | Issue: | 2 | Abstract: | Propolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Cell viability was assessed by resazurin assay, antioxidant activity was evaluated by DPPH assay, superoxide dismutase (SOD) 1 and 2 activity was evaluated by colorimetric assay. CD86 expression was determined by flow cytometry. IL-1β and HMOX-1 were analysed by Western blot. Results: Propolis did not affect cell viability and exhibited a potent antioxidant activity. Propolis alone induced SOD 1 activity. LPS stimulated SOD2, concomitantly or not with propolis. In the presence of LPS, propolis induced a higher HMOX-1 expression. Propolis inhibited CD86 expression stimulated by DNFB. LPS induced pro-IL-1β expression and propolis did not affect its action. Conclusion: Propolis exhibited an antioxidant action in a cell-free system and in a cell based-model. Propolis exerted an anti-inflammatory/antiallergic action, probably due to its antioxidant activity. | URI: | https://hdl.handle.net/10316/100510 | ISSN: | 26670313 | DOI: | 10.1016/j.phyplu.2022.100231 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais |
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1-s2.0-S2667031322000173-main.pdf | 3.69 MB | Adobe PDF | View/Open |
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