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Title: Exploring the antioxidant, anti-inflammatory and antiallergic potential of Brazilian propolis in monocytes
Authors: Conte, Fernanda Lopes
Pereira, Ana Catarina 
Brites, Gonçalo 
Ferreira, Isabel 
Silva, Ana Cristina 
Sebastião, Ana Isabel 
Matos, Patrícia 
Pereira, Cláudia 
Batista, Maria Teresa 
Sforcin, José Maurício 
Cruz, Maria Teresa 
Keywords: Anti-inflammatory action; Antiallergic action; Antioxidant activity; Monocyte; Propolis
Issue Date: 2022
Project: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/Programa Institucional de Internacionalização (CAPES/PRINT) under grant number: 88887.467268/2019–00 
Serial title, monograph or event: Phytomedicine Plus
Volume: 2
Issue: 2
Abstract: Propolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Cell viability was assessed by resazurin assay, antioxidant activity was evaluated by DPPH assay, superoxide dismutase (SOD) 1 and 2 activity was evaluated by colorimetric assay. CD86 expression was determined by flow cytometry. IL-1β and HMOX-1 were analysed by Western blot. Results: Propolis did not affect cell viability and exhibited a potent antioxidant activity. Propolis alone induced SOD 1 activity. LPS stimulated SOD2, concomitantly or not with propolis. In the presence of LPS, propolis induced a higher HMOX-1 expression. Propolis inhibited CD86 expression stimulated by DNFB. LPS induced pro-IL-1β expression and propolis did not affect its action. Conclusion: Propolis exhibited an antioxidant action in a cell-free system and in a cell based-model. Propolis exerted an anti-inflammatory/antiallergic action, probably due to its antioxidant activity.
ISSN: 26670313
DOI: 10.1016/j.phyplu.2022.100231
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais

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