Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8359
DC FieldValueLanguage
dc.contributor.authorRytter, Anna-
dc.contributor.authorCardoso, Carla M. P.-
dc.contributor.authorJohansson, Petra-
dc.contributor.authorCronberg, Tobias-
dc.contributor.authorHansson, Magnus J.-
dc.contributor.authorMattiasson, Gustav-
dc.contributor.authorElmér, Eskil-
dc.contributor.authorWieloch, Tadeusz-
dc.date.accessioned2009-02-09T14:51:24Z-
dc.date.available2009-02-09T14:51:24Z-
dc.date.issued2005en_US
dc.identifier.citationJournal of Neurochemistry. 95:4 (2005) 1108-1117en_US
dc.identifier.urihttp://hdl.handle.net/10316/8359-
dc.description.abstractThe aggravating effect of hyperglycemia on ischemic brain injury can be mimicked in a model of in vitro ischemia (IVI) using murine hippocampal slice cultures. Using this model, we found that the damage in the CA1 region following IVI in the absence or presence of 40 mm glucose (hyperglycemia) is highly temperature dependent. Decreasing the temperature from 35 to 31°C during IVI prevented cell death, whereas increasing the temperature by 2°C markedly aggravated damage. As blockade of the mitochondrial permeability transition (MPT) is equally effective as hypothermia in preventing ischemic cell death in vivo, we investigated whether inhibition of MPT or of caspases was protective following IVI. In the absence of glucose, the MPT blockers cyclosporin A and MeIle4-CsA but not the immunosuppressive compound FK506 diminished cell death. In contrast, following hyperglycemic IVI, MPT blockade was ineffective. Also, the pan-caspase inhibitor Boc-Asp(OMe)fluoromethyl ketone did not decrease cell death in the CA1 region following IVI or hyperglycemic IVI. We conclude that cell death in the CA1 region of organotypic murine hippocampal slices following IVI is highly temperature dependent and involves MPT. In contrast, cell death following hyperglycemic IVI, although completely prevented by hypothermia, is not mediated by mechanisms that involve MPT or caspase activation.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleThe temperature dependence and involvement of mitochondria permeability transition and caspase activation in damage to organotypic hippocampal slices following in vitro ischemiaen_US
dc.typearticleen_US
dc.identifier.doi10.1111/j.1471-4159.2005.03420.xen_US
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
obra.pdf440.97 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

14
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations

12
checked on May 29, 2020

Page view(s) 50

426
checked on Jul 8, 2020

Download(s) 10

1,092
checked on Jul 8, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.