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Title: Intracellular lithium and cyclic AMP levels are mutually regulated in neuronal cells
Authors: Montezinho, L. P. 
Duarte, C. B. 
Fonseca, C. P. 
Glinka, Y. 
Layden, B. 
Freitas, D. Mota de 
Geraldes, C. F. G. C. 
Castro, M. M. C. A. 
Issue Date: 2004
Citation: Journal of Neurochemistry. 90:4 (2004) 920-930
Abstract: In this work, we studied the effect of intracellular 3',5'-cyclic adenosine monophosphate (cAMP) on Li+ transport in SH-SY5Y cells. The cells were stimulated with forskolin, an adenylate cyclase activator, or with the cAMP analogue, dibutyryl-cAMP. It was observed that under forskolin stimulation both the Li+ influx rate constant and the Li+ accumulation in these cells were increased. Dibutyryl-cAMP also increased Li+ uptake and identical results were obtained with cortical and hippocampal neurons. The inhibitor of the Na+/Ca2+ exchanger, KB-R7943, reduced the influx of Li+ under resting conditions, and completely inhibited the effect of forskolin on the accumulation of the cation. Intracellular Ca2+ chelation, or inhibition of N-type voltage-sensitive Ca2+ channels, or inhibition of cAMP-dependent protein kinase (PKA) also abolished the effect of forskolin on Li+ uptake. The involvement of Ca2+ on forskolin-induced Li+ uptake was confirmed by intracellular free Ca2+ measurements using fluorescence spectroscopy. Exposure of SH-SY5Y cells to 1 mm Li+ for 24 h increased basal cAMP levels, but preincubation with Li+, at the same concentration, decreased cAMP production in response to forskolin. To summarize, these results demonstrate that intracellular cAMP levels regulate the uptake of Li+ in a Ca2+-dependent manner, and indicate that Li+ plays an important role in the homeostasis of this second messenger in neuronal cells.
DOI: 10.1111/j.1471-4159.2004.02551.x
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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