Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5850
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dc.contributor.authorFernandes, Fábio-
dc.contributor.authorNeves, Patrícia-
dc.contributor.authorGameiro, Paula-
dc.contributor.authorLoura, Luís M. S.-
dc.contributor.authorPrieto, Manuel-
dc.date.accessioned2008-09-26T17:42:52Z-
dc.date.available2008-09-26T17:42:52Z-
dc.date.issued2007en_US
dc.identifier.citationBiochimica et Biophysica Acta (BBA) - Biomembranes. 1768:11 (2007) 2822-2830en_US
dc.identifier.urihttp://hdl.handle.net/10316/5850-
dc.description.abstractThe outer membrane protein F (OmpF) is known to play an important role in the uptake of fluoroquinolone antibiotics by bacteria. In this study, the degree of binding of the fluoroquinolone antibiotic ciprofloxacin to OmpF in a lipid membrane environment is quantified using a methodology based on Förster resonance energy transfer (FRET). Analysis of the fluorescence quenching of OmpF is complex as each OmpF monomer presents two tryptophans at different positions, thus sensing two different distributions of acceptors in the bilayer plane. Specific FRET formalisms were derived accounting for the different energy transfer contributions to quenching of each type of tryptophan of OmpF, allowing the recovery of upper and lower boundaries for the ciprofloxacin-OmpF binding constant (KB). log (KB) was found to lie in the range 3.15-3.62 or 3.58-4.00 depending on the location for the ciprofloxacin binding site assumed in the FRET modelling, closer to the centre or to the periphery of the OmpF trimer, respectively. This methodology is suitable for the analysis of FRET data obtained with similar protein systems and can be readily adapted to different geometries.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T1T-4PCPFM2-2/1/3947bd4e257b7a6021d5f6b559a91dc3en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFluorescenceen_US
dc.subjectFluoroquinolonesen_US
dc.subjectMembrane proteinen_US
dc.subjectMembrane model systemen_US
dc.titleCiprofloxacin interactions with bacterial protein OmpF: Modelling of FRET from a multi-tryptophan protein trimeren_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculdade de Farmácia, Universidade de Coimbra-
crisitem.author.researchunitCoimbra Chemistry Center-
crisitem.author.orcid0000-0002-1051-2312-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
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