Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5053
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dc.contributor.authorBarsan, Madalina M.-
dc.contributor.authorKlincar, Janja-
dc.contributor.authorBatic, Martin-
dc.contributor.authorBrett, Christopher M.A.-
dc.date.accessioned2008-09-01T15:03:08Z-
dc.date.available2008-09-01T15:03:08Z-
dc.date.issued2007en_US
dc.identifier.citationTalanta. 71:5 (2007) 1893-1900en_US
dc.identifier.urihttp://hdl.handle.net/10316/5053-
dc.description.abstractA flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6THP-4M0BH9Y-2/1/306f5db86217ea276bf808fb05c02886en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFlow cellen_US
dc.subjectElectrochemical enzyme biosensoren_US
dc.subjectEnzyme immobilisationen_US
dc.subjectSol-gelen_US
dc.subjectGlucose determinationen_US
dc.subjectGrape musten_US
dc.titleDesign and application of a flow cell for carbon-film based electrochemical enzyme biosensorsen_US
dc.typearticleen_US
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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