Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5053
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Barsan, Madalina M. | - |
dc.contributor.author | Klincar, Janja | - |
dc.contributor.author | Batic, Martin | - |
dc.contributor.author | Brett, Christopher M. A. | - |
dc.date.accessioned | 2008-09-01T15:03:08Z | - |
dc.date.available | 2008-09-01T15:03:08Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Talanta. 71:5 (2007) 1893-1900 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5053 | - |
dc.description.abstract | A flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6THP-4M0BH9Y-2/1/306f5db86217ea276bf808fb05c02886 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Flow cell | en_US |
dc.subject | Electrochemical enzyme biosensor | en_US |
dc.subject | Enzyme immobilisation | en_US |
dc.subject | Sol-gel | en_US |
dc.subject | Glucose determination | en_US |
dc.subject | Grape must | en_US |
dc.title | Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.talanta.2006.08.032 | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0002-8492-5558 | - |
crisitem.author.orcid | 0000-0002-1972-4434 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
file751f479d18694aad93df1161e652ae80.pdf | 469.5 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
27
checked on Mar 18, 2024
WEB OF SCIENCETM
Citations
28
checked on Feb 2, 2024
Page view(s) 50
381
checked on Mar 26, 2024
Download(s)
275
checked on Mar 26, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.