Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/4811
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dc.contributor.authorSantos, D. A.-
dc.contributor.authorSalgado, Audrey I.-
dc.contributor.authorCunha, Rodrigo A.-
dc.date.accessioned2008-09-01T14:14:52Z-
dc.date.available2008-09-01T14:14:52Z-
dc.date.issued2003en_US
dc.identifier.citationNeuroscience Letters. 338:3 (2003) 225-228en_US
dc.identifier.urihttp://hdl.handle.net/10316/4811-
dc.description.abstractNerve stimulation increases the concentration of ATP in the synaptic cleft, which can act as a neurotransmitter or as a presynaptic neuromodulator. Using the luciferin-luciferase assay, we observed that the extracellular concentration of ATP increased by 11-26 nM over a basal concentration of 6 nM, in a frequency dependent manner (1-5 Hz), in the adult rat phrenic nerve-hemidiaphragm preparation. This ATP release depends on nerve activity since it was abolished by tetrodotoxin (1 [mu]M) and is strictly dependent on the presence of extracellular calcium. However, more than half of this nerve-evoked release of ATP is derived from activated muscle fibres since the selective post-synaptic nicotinic receptor antagonist, [alpha]-bungarotoxin (1 [mu]M), inhibited by over 60% the evoked release of ATP. The presently observed post-synaptic release of ATP together with the previously reported lack of post-synaptic effects of ATP and to the ability of ATP to act as a presynaptic modulator open the possibility that ATP may behave as a retrograde messenger at this neuromuscular junction.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T0G-47S6YPN-X/1/e09fe63a6ed4383fc2ed5996ece6ac41en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectReleaseen_US
dc.subjectNeuromuscular junctionen_US
dc.subjectPost-synapticen_US
dc.subjectPresynapticen_US
dc.titleATP is released from nerve terminals and from activated muscle fibres on stimulation of the rat phrenic nerveen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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