Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/4811
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Santos, D. A. | - |
dc.contributor.author | Salgado, Audrey I. | - |
dc.contributor.author | Cunha, Rodrigo A. | - |
dc.date.accessioned | 2008-09-01T14:14:52Z | - |
dc.date.available | 2008-09-01T14:14:52Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Neuroscience Letters. 338:3 (2003) 225-228 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/4811 | - |
dc.description.abstract | Nerve stimulation increases the concentration of ATP in the synaptic cleft, which can act as a neurotransmitter or as a presynaptic neuromodulator. Using the luciferin-luciferase assay, we observed that the extracellular concentration of ATP increased by 11-26 nM over a basal concentration of 6 nM, in a frequency dependent manner (1-5 Hz), in the adult rat phrenic nerve-hemidiaphragm preparation. This ATP release depends on nerve activity since it was abolished by tetrodotoxin (1 [mu]M) and is strictly dependent on the presence of extracellular calcium. However, more than half of this nerve-evoked release of ATP is derived from activated muscle fibres since the selective post-synaptic nicotinic receptor antagonist, [alpha]-bungarotoxin (1 [mu]M), inhibited by over 60% the evoked release of ATP. The presently observed post-synaptic release of ATP together with the previously reported lack of post-synaptic effects of ATP and to the ability of ATP to act as a presynaptic modulator open the possibility that ATP may behave as a retrograde messenger at this neuromuscular junction. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T0G-47S6YPN-X/1/e09fe63a6ed4383fc2ed5996ece6ac41 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Release | en_US |
dc.subject | Neuromuscular junction | en_US |
dc.subject | Post-synaptic | en_US |
dc.subject | Presynaptic | en_US |
dc.title | ATP is released from nerve terminals and from activated muscle fibres on stimulation of the rat phrenic nerve | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0304-3940(02)01419-2 | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-2550-6422 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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file4372e4c64f5d4761a58c9f6030d422a4.pdf | 92.89 kB | Adobe PDF | View/Open |
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