Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/47555
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dc.contributor.authorMota, Paula C.-
dc.contributor.authorTavares, Renata S.-
dc.contributor.authorCordeiro, Marília-
dc.contributor.authorPereira, Susana P.-
dc.contributor.authorPublicover, Stephen J.-
dc.contributor.authorOliveira, Paulo J.-
dc.contributor.authorRamalho-Santos, João-
dc.date.accessioned2018-02-12T13:26:42Z-
dc.date.available2018-02-12T13:26:42Z-
dc.date.issued2012-
dc.identifier.urihttps://hdl.handle.net/10316/47555-
dc.description.abstractObjective: The goal of this study was to verify if 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) could have any effect on male germ cells mitochondria and in this way add new insights in how male reproductive alterations observed in other studies occur. Methods: In vivo and in vitro approaches using rat testis and human sperm as models were employed to evaluate TCDD effects on testicular and sperm mitochondria after 24 h of exposure. Results: Testicular mitochondria from TCDD-treated rats presented no differences in the bioenergetic parameters monitored except for a significantly higher electric membrane potential in the presence of ADP, corroborated when TCDD was directly added to testicular mitochondria from untreated rats. Nevertheless, sperm mitochondrial membrane potential, motility, viability, capacitation and acrosomal integrity did not change after TCDD treatment. Moreover, only few sperm cells exposed to TCDD increased their intracellular Ca2+ concentration. Conlusions: TCDD can interact directly with rat testicular mitochondria inducing small changes. This effect, however, does not seem to occur in human sperm or it may be insufficient to induce significant alterations as observed by the maintenance of sperm function. 1.por
dc.description.sponsorshipP.C.M., R.S.T. and S.P.P. were supported by Portuguese FCT fellowships (SFRH/BD/23643/2005 and SFRH/BPD/74252/2010, SFRH/ BD/46002/2008 and SFRH/BD/64247/2009, respectively). CNC is supported by PEst-C/SAU/LA0001/2011, FCT. Referencespor
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subject2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)por
dc.subjectmitochondriapor
dc.subjectrat testispor
dc.subjecthuman spermpor
dc.subjectmale fertilitypor
dc.titleAcute effects of TCDD administration: special emphasis on testicular and sperm mitochondrial functionpor
dc.typearticle-
degois.publication.firstPage269por
degois.publication.lastPage276por
degois.publication.issue4por
degois.publication.titleAsian Pacific Journal of Reproductionpor
dc.peerreviewedyespor
dc.identifier.doi10.1016/S2305-0500(13)60091-3por
degois.publication.volume1por
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-9374-3476-
crisitem.author.orcid0000-0002-1168-2444-
crisitem.author.orcid0000-0002-5201-9948-
crisitem.author.orcid0000-0002-1172-4018-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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