Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/4690
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dc.contributor.authorÁlvaro, A. R.-
dc.contributor.authorMartins, J.-
dc.contributor.authorCosta, A. C.-
dc.contributor.authorFernandes, E.-
dc.contributor.authorCarvalho, F.-
dc.contributor.authorAmbrósio, A. F.-
dc.contributor.authorCavadas, C.-
dc.date.accessioned2008-09-01T14:12:48Z-
dc.date.available2008-09-01T14:12:48Z-
dc.date.issued2008en_US
dc.identifier.citationNeuroscience. 152:1 (2008) 97-105en_US
dc.identifier.urihttp://hdl.handle.net/10316/4690-
dc.description.abstractEcstasy (3,4-methylenedioxymethamphetamine; MDMA) has potent CNS stimulant effects. Besides the acute effects of MDMA, such as psychomotor activation, euphoria, decreased appetite, and hyperthermia, long-term damage of dopaminergic and serotonergic nerve terminals in multiple brain areas have also been reported. Although some studies have demonstrated that considerable amounts of MDMA reach the vitreous humor of the eye, and that serious visual consequences can result from MDMA consumption, the toxic effect of MDMA on the retina has not been completely elucidated. Neuropeptide Y (NPY) is present in the CNS, including the retina. The aim of the present study was to evaluate the effect of MDMA on rat retinal neural cell viability and investigate the involvement of 5-HT 2A-receptor (5-HT2A) activation. Moreover, the neuroprotective role of NPY on MDMA-induced toxicity was also investigated. MDMA induced necrosis [MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and propidium iodide assays] and apoptosis (immunoreactivity of cleaved caspase-3) in mixed cultures of retinal neural cells (neurons, macroglia and microglia), in a concentration-dependent manner. MDMA-induced toxicity was enhanced at higher temperature (40 °C) and was reduced by the 5HT2A-receptor antagonist, ketanserin (1 [mu]M). Interestingly, necrotic and apoptotic cell death induced by MDMA was inhibited by NPY (100 nM).en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T0F-4RDS1WF-1/1/a468528fe770ad17b607773d0d0f415aen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjecttoxicityen_US
dc.subjectapoptosisen_US
dc.subjectneuroprotectionen_US
dc.titleNeuropeptide Y protects retinal neural cells against cell death induced by ecstasyen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.neuroscience.2007.12.027-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculty of Medicine-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-0477-1641-
crisitem.author.orcid0000-0001-8020-9266-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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