Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/45046
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dc.contributor.authorBraga, Susana S.-
dc.contributor.authorMarques, Joana-
dc.contributor.authorHeister, Elena-
dc.contributor.authorDiogo, Cátia V.-
dc.contributor.authorOliveira, Paulo J.-
dc.contributor.authorPaz, Filipe A. Almeida-
dc.contributor.authorSantos, Teresa M.-
dc.contributor.authorMarques, Maria Paula M.-
dc.date.accessioned2017-12-15T16:17:55Z-
dc.date.available2017-12-15T16:17:55Z-
dc.date.issued2014-01-01-
dc.identifier10.1007/s10534-014-9725-8-
dc.identifier.urihttp://hdl.handle.net/10316/45046-
dc.description.abstractThe complex [Ru[9]aneS3(pdon)Cl]Cl (pdon = 1,10-phenanthroline-5,6-dione) was readily obtained from the stoichiometric reaction of Ru[9]aneS3(dmso)Cl2 with pdon. Recrystallisation in ethanol using salicylic acid as a co-crystallisation helper afforded single-crystals suitable for the collection of X-ray diffraction data which afforded a reasonable structural description. Two different kinds of molecular carriers were tested as vehicles for this complex: carbon nanotubes (CNTs) and cyclodextrins. CNTs had an insufficient loading rate for the ruthenium complex at CNT concentrations deemed non-cytotoxic on cultured cells. The cyclodextrin (CD) carriers, β-CD and TRIMEB (standing for permethylated β-CD), were able to form two adducts, studied by powder X-ray diffraction, thermogravimetric analysis (TGA), (13)C{(1)H} CP/MAS NMR and FT-IR spectroscopies. The DNA thermal denaturation studies showed that the complex 1 is able to intercalate with DNA. The in vitro cytotoxicity of the free complex [Ru[9]aneS3(pdon)Cl]Cl (1) and of its two CD adducts (2 and 3) was assessed on both rodent and human cell lines. By using the mouse K1735-M2 melanoma cell line and the non-tumour rat H9c2 cardiomyoblasts, the results showed that 1 and 2 significantly inhibited the growth of the tumour cell line while displaying a good safety profile on cardiomyoblasts. Compound 3 at 100 μM inhibited the proliferation of both cell lines, with a higher activity towards the melanoma cell line. The cytotoxicity of the compounds 1-3 was further assessed on human breast cancer cell lines. Against the MDA-MB-231 line, growth inhibition occurred only with 1 and 3 at the incubation time of 96 h, both with approximate inhibition rates of 50 %; against the MCF-7 line, mild cytotoxicity was observed at 48 h of incubation, with IC50 values calculated above 100 μM for 1, 2 and 3.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectAntineoplastic Agentspor
dc.subjectCell Survivalpor
dc.subjectCoordination Complexespor
dc.subjectCrystallography, X-Raypor
dc.subjectCyclodextrinspor
dc.subjectDNApor
dc.subjectDrug Carrierspor
dc.subjectDrug Screening Assays, Antitumorpor
dc.subjectHumanspor
dc.subjectInhibitory Concentration 50por
dc.subjectMCF-7 Cellspor
dc.subjectMelanomapor
dc.subjectMicepor
dc.subjectModels, Molecularpor
dc.subjectMolecular Conformationpor
dc.subjectMyoblasts, Cardiacpor
dc.subjectNanocapsulespor
dc.subjectNanotubes, Carbonpor
dc.subjectPowder Diffractionpor
dc.subjectRatspor
dc.subjectThermogravimetrypor
dc.subjectTransition Temperaturepor
dc.titleCarriers for metal complexes on tumour cells: the effect of cyclodextrins vs CNTs on the model guest phenanthroline-5,6-dione trithiacyclononane ruthenium(II) chloridepor
dc.typearticle-
degois.publication.firstPage507por
degois.publication.lastPage525por
degois.publication.issue3por
degois.publication.titleBioMetalspor
dc.peerreviewedyespor
dc.identifier.doi10.1007/s10534-014-9725-8por
degois.publication.volume27por
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitMolecular Physical-Chemistry-
crisitem.author.orcid0000-0002-8391-0055-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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