Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/37137
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dc.contributor.authorZaouali, Mohamed-
dc.contributor.authorPanisello, Arnau-
dc.contributor.authorLopez, Alexandre-
dc.contributor.authorCastro, Carlos-
dc.contributor.authorFolch, Emma-
dc.contributor.authorCarbonell, Teresa-
dc.contributor.authorRolo, Anabela-
dc.contributor.authorPalmeira, Carlos-
dc.contributor.authorGarcia-Gil, Agustin-
dc.contributor.authorAdam, René-
dc.contributor.authorRoselló-Catafau, Joan-
dc.date.accessioned2017-03-14T10:40:28Z-
dc.date.available2017-03-14T10:40:28Z-
dc.date.issued2017-
dc.identifier10.3390/ijms18030591-
dc.identifier.issn1422-0067por
dc.identifier.urihttp://hdl.handle.net/10316/37137-
dc.description.abstractWe investigated the involvement of glycogen synthase kinase-3β (GSK3β) and the voltage-dependent anion channel (VDAC) in livers subjected to cold ischemia-reperfusion injury (I/R) associated with orthotopic liver transplantation (OLT). Rat livers were preserved in University of Wisconsin (UW) and Institute Georges Lopez (IGL-1) solution, the latter enriched or not with trimetazidine, and then subjected to OLT. Transaminase (ALT) and HMGB1 protein levels, glutamate dehydrogenase (GLDH), and oxidative stress (MDA) were measured. The AKT protein kinase and its direct substrates, GSK3β and VDAC, as well as caspases 3, 9, and cytochrome C and reticulum endoplasmic stress-related proteins (GRP78, pPERK, ATF4, and CHOP), were determined by Western blot. IGL-1+TMZ significantly reduced liver injury. We also observed a significant phosphorylation of AKT, which in turn induced the phosphorylation and inhibition of GSK3β. In addition, TMZ protected the mitochondria since, in comparison with IGL-1 alone, we found reductions in VDAC phosphorylation, apoptosis, and GLDH release. All these results were correlated with decreased ER stress. Addition of TMZ to IGL-1 solution increased the tolerance of the liver graft to I/R injury through inhibition of GSK3β and VDAC, contributing to ER stress reduction and cell death prevention.por
dc.language.isoengpor
dc.publisherMDPIpor
dc.rightsopenAccesspor
dc.titleGSK3β and VDAC Involvement in ER Stress and Apoptosis Modulation during Orthotopic Liver Transplantationpor
dc.typearticle-
degois.publication.firstPage591por
degois.publication.issue3por
degois.publication.titleInternational Journal of Molecular Sciencespor
dc.relation.publisherversionhttp://www.mdpi.com/journal/ijmspor
dc.peerreviewedyespor
dc.identifier.doi10.3390/ijms18030591por
degois.publication.volume18por
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0003-3535-9630-
crisitem.author.orcid0000-0002-2639-7697-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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