Please use this identifier to cite or link to this item:
Title: Tooth discolouration analysis induced by two Calcium Silicate-based Materials: in vitro study
Authors: Nascimento, Ana Rita Bernardes 
Orientador: Palma, Paulo
Ramos, João Carlos
Keywords: Tooth discolouration; Tooth staining,; White MTA; Biodentine; Spectophometry; CIE colour system
Issue Date: 2013
Abstract: Aim: to evaluate the over time chromatic alterations/discolouration in tooth crowns induced by two Calcium silicate based-materials (CSMs) - White mineral trioxide aggregate (WMTA- ProRoot® MTA) and BiodentineTM - used to fill pulp chambers. Materials and Methods: Twenty-eight premolar human teeth were sectioned 2 mm below cemento-enamel junction (CEJ). Pulp tissues were extirpated through the cervical cut, and occlusal standardized cavities were prepared. The specimens were split according to a stratified random sampling by value into four groups: Group 1- Negative control (filled with dry sterile cotton pellets) (n=4); Group 2- Positive control (filled with blood moistened sterile cotton pellets) (n=4); Group 3- WMTA (ProRoot® MTA) (n=10); Group 4- Biodentine™ (n=10). Colour measurements were recorded with Colorimeter (PR®-650 SpectraScan® Colorimeter, Topanga Canyon Place Chatsworth, CA) at 3 time points: T0, baseline; T1, immediately after material placement, and T2, 6 weeks after. The measurements were taken under standardized conditions: dark room and a white cardboard box illuminated with a LED light of 5500k and 6 watts. Data were transformed into values of the CIE L*a*b* colour system, and the corresponding colour differences (ΔE) values were calculated. Between the measurements the specimens were stored in tap water, in the dark, in a 100% humidity environment at 37°C, with normal atmospheric gas levels. The results were analysed using repeated ANOVA measurements, unilateral t-Student test considering a cut point of 2.3, mixed ANOVA procedures, Kruskal-Wallis test, Mann- Whitney test, and one-way ANOVA with Tukey post-hoc tests. The significance level was set at α<0.05. Results: There were not found statistically significant differences in colour alteration (ΔE) between the two CMSs (group 3-WMTA and 4-Biodentine™), for all time intervals. Relative to L* parameter, it is possible to observe statistically significant differences, between WMTA and Biodentine™. Conclusion: Concerning global colour variation (ΔE), there was no significant difference between the two CMSs (WMTA and Biodentine™), for all time intervals, over time. However, relative to L* parameter, it was possible to observe statistically significant differences, between WMTA and Biodentine™, being the L* variation for WMTA higher, indicating a greater tooth discolouration in terms of value.
Description: Trabalho final do 5º ano com vista à atribuição do grau de mestre no âmbito do ciclo de estudos de Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina da Universidade de Coimbra.
Rights: openAccess
Appears in Collections:FMUC Med. Dentária - Teses de Mestrado
UC - Dissertações de Mestrado

Files in This Item:
File Description SizeFormat
FINAL Rita (1).pdfFINAL Rita (1)4.57 MBAdobe PDFView/Open
Show full item record

Page view(s)

checked on Sep 19, 2022

Download(s) 20

checked on Sep 19, 2022

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.