Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/28266
Title: Membrane progesterone receptors in human regulatory T cells: a reality in pregnancy
Authors: Areia, Ana 
Vale-Pereira, Sofia 
Alves, Vera 
Rodrigues-Santos, Paulo 
Moura, Paulo 
Mota-Pinto, Anabela 
Keywords: Pregnancy; Progesterone receptor; T regulatory cells
Issue Date: 2-Feb-2015
Publisher: Royal College of Obstetricians and Gynaecologists
Serial title, monograph or event: BJOG: An International Journal of Obstetrics & Gynaecology
Abstract: Objective - To provide evidence of the existence of membrane progesterone receptor alpha (mPRa) on regulatory T cells (Treg) in peripheral blood during pregnancy, postulating a possible explanation for the effect of progesterone on preterm birth. Design Cross-sectional study. Setting - Tertiary Obstetric Department in a University Hospital. Population - Healthy pregnant women. Methods - Treg cells from peripheral blood samples were studied by flow cytometry using multiple monoclonal antibody expression. Main outcome measures - Evaluate the number and percentage of CD4+CD25highCD127low, the number and percentage of Treg cells among the total CD4+ T cells, and the percentage and mean fluorescence intensity (MFI) of mPRa in that population, using several gating strategies. Results - 43 peripheral blood samples were collected from healthy women during pregnancy, whose median gestational age was 28.7 7.1 (16–40) weeks. The percentage of CD4+ in the total lymphocytes was 43% (32–51) and the percentage of CD4+CD25highCD127low was 4.8% (1.6–5.9), with only 45% (16–72) of those cells expressing the intracellular marker FoxP3 (Treg cell pool). We confirmed the existence of mPRa in that specific population because 8.0% (2.02–33) of the Treg cells were marked with the specific monoclonal antibody, with an mPRa+ MFI of 719 (590–1471). Conclusions - This research shows that Treg cells express mPRa during pregnancy, which might play an important role in immune modulation by progesterone.
URI: http://hdl.handle.net/10316/28266
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Membrane progesterone receptors(BJOG15).pdf217.14 kBAdobe PDFView/Open
Show full item record

Page view(s) 50

386
checked on Jan 22, 2020

Download(s) 20

831
checked on Jan 22, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.