Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/26859
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dc.contributor.authorVicentini, Fernando Campanhã-
dc.contributor.authorJanegitz, Bruno C.-
dc.contributor.authorBrett, Christopher M. A.-
dc.contributor.authorFatibello-Filho, Orlando-
dc.date.accessioned2014-09-23T13:22:59Z-
dc.date.available2014-09-23T13:22:59Z-
dc.date.issued2013-11-
dc.identifier.citationVICENTINI, Fernando Campanhã [et al.] - Tyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate film. "Sensors and Actuators B: Chemical". ISSN 0925-4005. Vol. 188 (2013) p. 1101-1108por
dc.identifier.issn0925-4005-
dc.identifier.urihttp://hdl.handle.net/10316/26859-
dc.description.abstractA glassy carbon electrode modified with functionalized multi-walled carbon nanotubes (MWCNT), 1-butyl-3-methylimidazolium chloride (ionic liquid = IL) and tyrosinase (Tyr) within a dihexadecylphosphate (DHP) film for the development of a biosensor is proposed. MWCNT, IL and Tyr were efficiently immobilized in the film using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) as crosslinking agents, and which was characterized by cyclic voltammetry (CV) in the presence of catechol. The IL-MWCNT nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, since the biosensor presented biocatalytic activity toward the oxidation of catechol to o-quinone which was electrochemically reduced to catechol at a potential of 0.04 V. The analytical curve ranged from 4.9 × 10−6 to 1.1 × 10−3 mol L−1 with a detection limit of 5.8 × 10−7 mol L−1. The developed Tyr-IL-MWCNT-DHP/GCE biosensor showed a wide linear range, good reproducibility, sensitivity and stability and the biosensor was successfully applied to the determination of catechol in natural water samples, with satisfactory results compared with a spectrophotometric method, at the 95% confidence level.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subject1-Butyl-3-methylimidazolium chloridepor
dc.subjectCatechol determinationpor
dc.subjectDihexadecylphosphatepor
dc.subjectIonic liquidpor
dc.subjectMulti-walled carbon nanotubespor
dc.subjectTyrosinase biosensorpor
dc.titleTyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate filmpor
dc.typearticlepor
degois.publication.firstPage1101por
degois.publication.lastPage1108por
degois.publication.titleSensors and Actuators B: Chemicalpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400513009143por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.snb.2013.07.109-
degois.publication.volume188por
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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