Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/25797
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dc.contributor.authorSobral, Olímpia-
dc.contributor.authorMarin-Morales, Maria Aparecida-
dc.contributor.authorRibeiro, R.-
dc.date.accessioned2014-05-28T11:14:22Z-
dc.date.available2014-05-28T11:14:22Z-
dc.date.issued2013-
dc.identifier.urihttp://hdl.handle.net/10316/25797-
dc.description.abstractContaminant driven genetic erosion reported through the inspection of selectable traits can be underestimated using neutral markers. This divergence was previously reported in the aquatic system of an abandoned pyrite mine. The most sensitive genotypes of the microcrustacean cladoceran Daphnia longispina were found to be lacking in the impacted reservoir near the entrance of the metal rich acid mine drainage (AMD). Since that divergence could be, at least partially, accounted for by mutagenicity and genotoxicity of the AMD, the present study aimed at providing such a characterization. The Allium cepa chromosomal aberration assay, using root meristematic cells, was carried out, by exposing seeds to 100, 10, 1, and 0.1 % of the local AMD. Chromosomal aberrations, cell division phases and cell death were quantified after the AMD exposure and after 24 and 48 h recovery periods. The AMD revealed to be mutagenic and genotoxic, even after diluting it to 1 and 0.1 %. Dilutions within this range were previously found to be below the lethality threshold and to elicit sublethal effects on reproduction of locally collected D. longispina clonal lineages Significant mutagenic effects (micronuclei and chromosomal breaks) were also found at 0.1 % AMD, supporting that exposure may induce permanent genetic alterations. Recovery tests showed that AMD genotoxic effects persisted after the exposure.por
dc.description.sponsorshipThis study was partially was funded by FSE and POPH by national funds through Fundação para a Ciência e a Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior (Ph.D. Grant SFRH/BD/28526/2006 to Olímpia Sobral).por
dc.language.isoengpor
dc.publisherSpringer Sciencepor
dc.rightsopenAccesspor
dc.subjectChromosomal aberrationspor
dc.subjectMicronucleipor
dc.subjectAllium cepapor
dc.subjectAcid mine drainagepor
dc.subjectRecoverypor
dc.titleCould contaminant induced mutations lead to a genetic diversity overestimation?por
dc.typearticlepor
degois.publication.firstPage838por
degois.publication.lastPage846por
degois.publication.issue5por
degois.publication.titleEcotoxicologypor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10646-013-1079-4por
dc.peerreviewedYespor
dc.identifier.doi10.1007/s10646-013-1079-4-
degois.publication.volume22por
uc.controloAutoridadeSim-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.orcid0000-0002-0883-1939-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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