Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/25575
DC FieldValueLanguage
dc.contributor.authorFigueiredo, Joana-
dc.contributor.authorSimões, Maria José-
dc.contributor.authorGomes, Paula-
dc.contributor.authorBarroso, Cristina-
dc.contributor.authorPinho, Diogo-
dc.contributor.authorConceição, I. L. P. M. da-
dc.contributor.authorFonseca, Luís-
dc.contributor.authorAbrantes, I. M. O.-
dc.contributor.authorPinheiro, Miguel-
dc.contributor.authorEgas, Conceição-
dc.date.accessioned2014-04-22T11:18:58Z-
dc.date.available2014-04-22T11:18:58Z-
dc.date.issued2013-
dc.identifier.urihttps://hdl.handle.net/10316/25575-
dc.description.abstractThe pinewood nematode, Bursaphelenchus xylophilus, is native to North America but it only causes damaging pine wilt disease in those regions of the world where it has been introduced. The accurate detection of the species and its dispersal routes are thus essential to define effective control measures. The main goals of this study were to analyse the genetic diversity among B. xylophilus isolates from different geographic locations and identify single nucleotide polymorphism (SNPs) markers for geographic origin, through a comparative transcriptomic approach. The transcriptomes of seven B. xylophilus isolates, from Continental Portugal (4), China (1), Japan (1) and USA (1), were sequenced in the next generation platform Roche 454. Analysis of effector gene transcripts revealed inter-isolate nucleotide diversity that was validated by Sanger sequencing in the genomic DNA of the seven isolates and eight additional isolates from different geographic locations: Madeira Island (2), China (1), USA (1), Japan (2) and South Korea (2). The analysis identified 136 polymorphic positions in 10 effector transcripts. Pairwise comparison of the 136 SNPs through Neighbor-Joining and the Maximum Likelihood methods and 5-mer frequency analysis with the alignment-independent bilinear multivariate modelling approach correlated the SNPs with the isolates geographic origin. Furthermore, the SNP analysis indicated a closer proximity of the Portuguese isolates to the Korean and Chinese isolates than to the Japanese or American isolates. Each geographic cluster carried exclusive alleles that can be used as SNP markers for B. xylophilus isolate identification.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.titleAssessment of the Geographic Origins of Pinewood Nematode Isolates via Single Nucleotide Polymorphism in Effector Genespor
dc.typearticlepor
degois.publication.firstPageArtigo e83542por
degois.publication.issue12por
degois.publication.titlePLoS ONEpor
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0083542por
dc.peerreviewedYespor
dc.identifier.doi10.1371/journal.pone.0083542-
degois.publication.volume8por
uc.controloAutoridadeSim-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.orcid0000-0002-1995-1231-
crisitem.author.orcid0000-0002-3017-0639-
crisitem.author.orcid0000-0001-7405-8916-
crisitem.author.orcid0000-0002-8761-2151-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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