Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/20324
DC FieldValueLanguage
dc.contributor.authorVale-Pereira, Sofia-
dc.contributor.authorTodo-Bom, Ana-
dc.contributor.authorGeraldes, Luisa-
dc.contributor.authorSchmidt-Weber, Carsten-
dc.contributor.authorAkdis, Cesmi-
dc.contributor.authorMota-Pinto, Anabela-
dc.date.accessioned2012-07-11T19:12:42Z-
dc.date.available2012-07-11T19:12:42Z-
dc.date.issued2011-
dc.identifier.urihttp://hdl.handle.net/10316/20324-
dc.description.abstractBackground Asthma is a chronic inflammatory disorder in which Th2, Th1 and suppressive T cells (Tregs) play a role. The transcription factor FoxP3 plays a role in Treg differentiation hile T-bet is important for Th1 and GATA-3 for Th2 differentiation from naïve T cells. Recent data show that age-related deregulation of Treg cells is a mechanism of senescence affecting several chronic diseases. It is crucial to understand the behaviour of these cell populations in asthma for elderly patients. Objective To evaluate FoxP3, GATA-3 and T-bet gene expression under basal conditions and after in vitro activation in a group of elderly asthmatic compared with age-matched healthy individuals. Methods Thirty-two elderly asthmatics and 17 healthy elderly individuals were selected. Serum total IgE was measured, and peripheral blood mononuclear cells (PBMCs) were isolated and stimulated in vitro with anti-CD3/anti-CD28, followed by mRNA isolation. After reverse transcription, real-time quantitative PCR was performed and relative quantification was determined 2 DDCt (2 DDCt method). Results The mean values and standard deviation of FoxP3, GATA-3 and T-bet relative expression for control vs. asthma were 10.2 6.8 vs. 4.8 3.8, 2.4 2.9 vs. 1.7 0.9 and 3.3 2.1 vs. 2.1 1.5, respectively. Healthy individuals showed significantly higher expression of FoxP3 and T-bet; asthmatics had a lower T-bet/GATA-3 ratio, higher serum IgE and a positive significant correlation between total IgE and GATA-3 expression. Conclusion and clinical relevance Elderly asthmatic patients have lower FoxP3 mRNA expression in PBMC, which can be associated with the sustained inflammatory process and with the decreased immune tolerance by Treg cells. The T-bet deficiency and the correlation of GATA-3 expression with the increase of IgE are characteristics of long-lasting asthma. Changes related to the immunosenescence process could provide an explanation for the minor differences observed between the groups. It is important to clarify persistent modifications in long-lasting asthma in the elderly and adequate future therapeutic approaches.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectFoxp3por
dc.subjectAsthmapor
dc.titleFoxP3, GATA-3 and T-bet expression in elderly asthmapor
dc.typearticlepor
degois.publication.firstPage490por
degois.publication.lastPage496por
degois.publication.issue4por
degois.publication.titleClinical & Experimental Allergypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2222.2010.03640.x/abstract;jsessionid=3EFE5AEAE7E92D796B2A846F20E01CB5.d03t02por
dc.peerreviewedYespor
dc.identifier.doi10.1111/j.1365-2222.2010.03640.x-
degois.publication.volume41por
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Medicina, Universidade de Coimbra-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0002-0820-9568-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
FoxP3, GATA-3 and T-bet expression in elderly asthma(ClinExpAllergy2011).pdf243.68 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

41
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations 5

38
checked on Oct 14, 2020

Page view(s)

258
checked on Oct 20, 2020

Download(s) 50

244
checked on Oct 20, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.