Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/11811
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dc.contributor.authorCruz, Maria Teresa-
dc.contributor.authorDuarte, Carlos B.-
dc.contributor.authorGonçalo, Margarida-
dc.contributor.authorFigueiredo, Américo-
dc.contributor.authorCarvalho, Arsélio P.-
dc.contributor.authorLopes, Maria Celeste-
dc.date.accessioned2009-10-23T15:00:12Z-
dc.date.available2009-10-23T15:00:12Z-
dc.date.issued2003-
dc.identifier.citationInternational Journal of Toxicology. 22 (2003) 43-48en_US
dc.identifier.issn1091-5818-
dc.identifier.urihttps://hdl.handle.net/10316/11811-
dc.description.abstractIn this work, a dendritic cell line derived from mouse skin (FSDC) was used, as an in vitro experimental model, to evaluate the cytotoxic effect of two chemical sensitizers, a strong sensitizer (2,4-dinitrofluorobenzene, DNFB) and a weak sensitizer (2,4- dichloronitrobenzene, DCNB). The results indicated that DNFB reduces the cellular metabolism of FSDC, as evaluated by the reduction of the tetrazolium salt, 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT). All the DNFB concentrations tested, ranging from 5.2 ¹M to 26¹M, significantly inhibited the MTT reduction after 1 hour of cell exposure to the sensitizer. In contrast, incubation of FSDC with the weak sensitizer DCNB had no significant effect on the MTT reduction assay. When the cells were incubated withDNFB(13¹M), for 3 and 6 hours, morphological changes characteristics of cell death by apoptosis were observed, as assessed by propidium iodide (PI) DNA staining and annexin-V externalization analysis. These results correlate well with an increase of caspase-3–like activity after FSDC exposure to DNFB (13 ¹M) for 6 hours. Together, these results indicate that apoptotic death of skin dendritic cells occurs after exposure to the sensitizer DNFB, although necrotic cell death was also observed when the cells were incubated with high concentrations of DNFB (26 ¹M), or after long periods of cell exposure to the chemicalDNFB(13¹M, for 6 hours).en_US
dc.language.isoengen_US
dc.publisherInforma Healthcareen_US
dc.rightsopenAccessen_US
dc.subjectApoptosisen_US
dc.subjectCaspase-3en_US
dc.subjectCell Deathen_US
dc.subjectDCNBen_US
dc.subjectDendritic Cellsen_US
dc.subjectDNFBen_US
dc.titleThe Sensitizer 2,4-Dinitrofluorobenzene Activates Caspase-3 and Induces Cell Death in a Skin Dendritic Cell Lineen_US
dc.typearticleen_US
dc.identifier.doi10.1080/10915810305069-
uc.controloAutoridadeSim-
item.openairetypearticle-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-9846-6754-
crisitem.author.orcid0000-0002-1474-0208-
crisitem.author.orcid0000-0001-6842-1360-
crisitem.author.orcid0000-0002-9105-9619-
crisitem.author.orcid0000-0002-6469-0894-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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