Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/101620
Title: From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
Authors: Simões, Tiago 
Novais, Sara C
Luz, Tiago Natal da 
Renaud, Mathieu 
Leston, Sara 
Ramos, Fernando 
Römbke, Jörg 
Roelofs, Dick
van Straalen, Nico M
Sousa, José P. 
Lemos, Marco Filipe Loureiro 
Keywords: Biomarkers; Chlorothalonil; Collembola; Fungicides; In situ validation; qPCR
Issue Date: 2019
Project: UID/MAR/04292/2019 
ENVIRONOME (PTDC/AGR-PRO/3496/2012 - POCI-01-0145-FEDER- 016773) 
SFRH/BD/98266/2013 
SFRH/BPD/94500/2013 
SFRH/BPD/110943/2015 
Integrated Programme of SR& TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) 
EU FP7 Sustainable Nanotechnologies Project (SUN, contract number 604305) 
Serial title, monograph or event: Environment International
Volume: 127
Abstract: Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.
URI: http://hdl.handle.net/10316/101620
ISSN: 01604120
DOI: 10.1016/j.envint.2019.03.073
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais

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