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https://hdl.handle.net/10316/101258
Title: | Myocardial infarction affects Cx43 content of extracellular vesicles secreted by cardiomyocytes | Authors: | Martins-Marques, Tânia Rodrigues, Teresa Ribeiro de Jager, Saskia C Zuzarte, Mónica Ferreira, Cátia Cruz, Pedro Reis, Liliana Baptista, Rui Gonçalves, Lino Sluijter, Joost Pg Girão, Henrique |
Issue Date: | 2020 | Project: | PAC “NETDIAMOND” POCI-01-0145-FEDER-016385 HealthyAging2020 CENTRO-01-0145-FEDER-000012-N2323; POCI-01-0145-FEDER-007440, CENTRO-01- 0145-FEDER-032179, CENTRO-01-0145-FEDER-032414, POCI-01-0145-FEDER-022122, FCTUID/NEU/04539/2013, UID/NEU/04539/2019, UIDB/04539/2020, and UIDP/ 04539/2020) Project EVICARE (No. 725229) of the European Research Council FCT - PD/BD/106043/2015 FCT - PD/BD/52294/2013 |
Serial title, monograph or event: | Life Science Alliance | Volume: | 3 | Issue: | 12 | Abstract: | Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular vesicle surface, mediating the release of vesicle content into target cells. Here, using a comprehensive strategy that included cell-based approaches, animal models and human patients, we demonstrate that myocardial ischemia impairs the secretion of Cx43 into circulating, intracardiac and cardiomyocyte-derived vesicles. In addition, we show that ubiquitin signals Cx43 release in basal conditions but appears to be dispensable during ischemia, suggesting an interplay between ischemia-induced Cx43 degradation and secretion. Overall, this study constitutes a step forward for the characterization of the signals and molecular players underlying vesicle protein sorting, with strong implications on long-range intercellular communication, paving the way towards the development of innovative diagnostic and therapeutic strategies for cardiovascular disorders. | URI: | https://hdl.handle.net/10316/101258 | ISSN: | 2575-1077 | DOI: | 33097557 2575-1077 33097557 2575-1077 2575-1077 33097557 10.26508/lsa.202000821 33097557 2575-1077 |
Rights: | openAccess |
Appears in Collections: | I&D ICBR - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais |
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