Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/101258
Title: Myocardial infarction affects Cx43 content of extracellular vesicles secreted by cardiomyocytes
Authors: Martins-Marques, Tânia 
Rodrigues, Teresa Ribeiro 
de Jager, Saskia C
Zuzarte, Mónica 
Ferreira, Cátia 
Cruz, Pedro 
Reis, Liliana
Baptista, Rui 
Gonçalves, Lino 
Sluijter, Joost Pg
Girão, Henrique 
Issue Date: 2020
Project: PAC “NETDIAMOND” POCI-01-0145-FEDER-016385 
HealthyAging2020 CENTRO-01-0145-FEDER-000012-N2323; POCI-01-0145-FEDER-007440, CENTRO-01- 0145-FEDER-032179, CENTRO-01-0145-FEDER-032414, POCI-01-0145-FEDER-022122, FCTUID/NEU/04539/2013, UID/NEU/04539/2019, UIDB/04539/2020, and UIDP/ 04539/2020) 
Project EVICARE (No. 725229) of the European Research Council 
FCT - PD/BD/106043/2015 
FCT - PD/BD/52294/2013 
Serial title, monograph or event: Life Science Alliance
Volume: 3
Issue: 12
Abstract: Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular vesicle surface, mediating the release of vesicle content into target cells. Here, using a comprehensive strategy that included cell-based approaches, animal models and human patients, we demonstrate that myocardial ischemia impairs the secretion of Cx43 into circulating, intracardiac and cardiomyocyte-derived vesicles. In addition, we show that ubiquitin signals Cx43 release in basal conditions but appears to be dispensable during ischemia, suggesting an interplay between ischemia-induced Cx43 degradation and secretion. Overall, this study constitutes a step forward for the characterization of the signals and molecular players underlying vesicle protein sorting, with strong implications on long-range intercellular communication, paving the way towards the development of innovative diagnostic and therapeutic strategies for cardiovascular disorders.
URI: http://hdl.handle.net/10316/101258
ISSN: 2575-1077
DOI: 33097557
2575-1077
33097557
2575-1077
2575-1077
33097557
10.26508/lsa.202000821
33097557
2575-1077
Rights: openAccess
Appears in Collections:I&D CIBB - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais

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