Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/10114
DC FieldValueLanguage
dc.contributor.authorKöfalvi, A.-
dc.contributor.authorPereira, M. F.-
dc.contributor.authorRebola, N.-
dc.contributor.authorRodrigues, R. J.-
dc.contributor.authorOliveira, C. R.-
dc.contributor.authorCunha, R. A.-
dc.date.accessioned2009-04-30T14:08:37Z-
dc.date.available2009-04-30T14:08:37Z-
dc.date.issued2007en_US
dc.identifier.citationBritish Journal of Pharmacology. 151:4 (2007) 551-563en_US
dc.identifier.urihttps://hdl.handle.net/10316/10114-
dc.description.abstractInhibitory CB1 cannabinoid receptors and excitatory TRPV1 vanilloid receptors are abundant in the hippocampus. We tested if two known hybrid endocannabinoid/endovanilloid substances, N-arachidonoyl-dopamine (NADA) and anandamide (AEA), presynapticaly increased or decreased intracellular calcium level ([Ca2+]i) and GABA and glutamate release in the hippocampus. Resting and K+-evoked levels of [Ca2+]i and the release of [3H]GABA and [3H]glutamate were measured in rat hippocampal nerve terminals. NADA and AEA per se triggered a rise of [Ca2+]i and the release of both transmitters in a concentration- and external Ca2+-dependent fashion, but independently of TRPV1, CB1, CB2, or dopamine receptors, arachidonate-regulated Ca2+-currents, intracellular Ca2+ stores, and fatty acid metabolism. AEA was recently reported to block TASK-3 potassium channels thereby depolarizing membranes. Common inhibitors of TASK-3, Zn2+, Ruthenium Red, and low pH mimicked the excitatory effects of AEA and NADA, suggesting that their effects on [Ca2+]i and transmitter levels may be attributable to membrane depolarization upon TASK-3 blockade. The K+-evoked Ca2+ entry and Ca2+-dependent transmitter release were inhibited by nanomolar concentrations of the CB1 receptor agonist WIN55212-2; this action was sensitive to the selective CB1 receptor antagonist AM251. However, in the low micromolar range, WIN55212-2, NADA and AEA inhibited the K+-evoked Ca2+ entry and transmitter release independently of CB1 receptors, possibly through direct Ca2+ channel blockade. We report here for hybrid endocannabinoid/endovanilloid ligands novel dual functions which were qualitatively similar to activation of CB1 or TRPV1 receptors, but were mediated through interactions with different targets. British Journal of Pharmacology (2007) 151, 5512013563; doi:en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleAnandamide and NADA bi-directionally modulate presynaptic Ca<sup>2+</sup> levels and transmitter release in the hippocampusen_US
dc.typearticleen_US
dc.identifier.doi10.1038/sj.bjp.0707252en_US
item.openairetypearticle-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-6942-4328-
crisitem.author.orcid0000-0003-2550-6422-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
fulltext.pdf388.72 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

40
checked on Nov 9, 2022

WEB OF SCIENCETM
Citations 5

31
checked on May 2, 2023

Page view(s) 50

576
checked on Jul 17, 2024

Download(s) 20

1,064
checked on Jul 17, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.