This work was funded by FEDER through the Program COMPETE and by Portuguese fund through FCT in context of the projects “AGING-MODEL” (Ref. POCI-01-0145- FEDER-029229) and “Unraveling the Rules of Passive Permeation Through the Blood-Brain Barrier” (Ref: PTDC/DTP-FTO/2784/2014), as well as the European project ERAatUC (ref. 669088). LF would like to thank Dr. Hugo Fernandes for the critical reading of the manuscript

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This work was funded by FEDER through the Program COMPETE and by Portuguese fund through FCT in context of the projects “AGING-MODEL” (Ref. POCI-01-0145- FEDER-029229) and “Unraveling the Rules of Passive Permeation Through the Blood-Brain Barrier” (Ref: PTDC/DTP-FTO/2784/2014), as well as the European project ERAatUC (ref. 669088). LF would like to thank Dr. Hugo Fernandes for the critical reading of the manuscript